Plant regeneration via somatic embryogenesis from root explants of Hevea brasiliensis

文献类型： 外文期刊

第一作者： Zhou, Quan-Nan

作者： Zhou, Quan-Nan;Huang, Tian-Dai;Li, Wei-Guo;Sun, Ai-Hua;Dai, Xue-Mei;Li, Zhe;Jiang, Ze-Hai

作者机构：

关键词： Hevea brasiliensis Muell. Arg.;root explants;callus;somatic embryogenesis;regeneration

期刊名称：AFRICAN JOURNAL OF BIOTECHNOLOGY （ 影响因子：0.573； 五年影响因子：0.794 ）

ISSN： 1684-5315

年卷期： 2010 年 9 卷 48 期

页码：

收录情况： SCI

摘要： A system for induction of callus and plant regeneration via somatic embryogenesis from root explants of Hevea brasiliensis Muell. Arg. clone Reyan 87-6-62 was evaluated. The influence of plant growth regulators (PGRs) including 2,4-dichlorophenoxyacetic acid (2,4-D), 6-benzylaminopurine (6-BA) and kinetin (KT) on callus induction of root explants from in vitro plantlets were studied. The results showed that the highest induction frequency of embryogenic callus emerged on Murashige and Skoog (MS) medium supplemented with 1 mg/l KT, 0.2 mg/l 6-BA without 2,4-D. Mean of 4 somatic embryos per embryogenic callus were obtained and approximately 11.8% of them developed into plantlets. The regenerated plantlets were successfully transplanted to sand bed. The plant regeneration system established in this study will facilitate mass propagation and may be applied to culture the roots of high-yielding rubber trees.

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