Combined amino acids modulation with H2O2 stress for glutathione overproduction in Candida utilis

文献类型： 外文期刊

第一作者： Xie, Jianghui

作者： Xie, Jianghui;Mo, Yiwei;Wang, Bin;Liang, Guobin;Zhou, Quanfa

作者机构：

关键词： Amino acids;glutathione (GSH);high cell density (HCD) cultivation;Candida utilis;H2O2 stresses

期刊名称：AFRICAN JOURNAL OF BIOTECHNOLOGY （ 影响因子：0.573； 五年影响因子：0.794 ）

ISSN： 1684-5315

年卷期： 2010 年 9 卷 33 期

页码：

收录情况： SCI

摘要： Strategies of amino acids addition coupled with H2O2 stresses were developed for glutathione (GSH) overproduction in high cell density (HCD) cultivation of Candida utilis. Based on the fact that glycine shows two functions of promoting cells growth as well as GSH production, precursor amino acids modulations of feeding glycine at 4 mmol/l/h at exponential phase and adding precursor amino acids (glutamic acid 42 mmol/l, glycine 40 mmol/l, and cysteine 36 mmol/) at stationary phase were conducted. As a result, cell density reached 114.8 g/l at 45 h and glutathione yield of 2136 mg/l was achieved at 60 h, which was 12.5 and 90.2% higher than the control, respectively. Furthermore, the novel strategies of amino acids modulation combined with H2O2 additions (24 mmol/l at 21 h, 26 mmol/l at 29 h, 28 mmol/l at 37 h and 30 mmol/l at 45 h) were adopted to maximize glutathione production. Final glutathione yield reached 2448 mg/l after 60 h cultivation, suggesting the strategies developed as being feasible for GSH overproduction.

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