Protein interaction matrix of Papaya ringspot virus type P based on a yeast two-hybrid system
文献类型: 外文期刊
第一作者: Shen, W. T.
作者: Shen, W. T.;Wang, M. Q.;Yan, P.;Gao, L.;Zhou, P.
作者机构:
关键词: Potyvirus;protein-protein interaction;Papaya ringspot virus;yeast two-hybrid system
期刊名称:ACTA VIROLOGICA ( 影响因子:1.162; 五年影响因子:1.255 )
ISSN: 0001-723X
年卷期: 2010 年 54 卷 1 期
页码:
收录情况: SCI
摘要: Comprehensive analysis of the interactions between 10 mature proteins of Papaya ringspot virus type P (PRSV-P) was carried out based on a yeast two-hybrid system assay (YTHS). We detected 6 interactions between different viral proteins (VPg-P1, VPg-P3, VPg-CI, VPg-CP, NIaPro-CI, and Nlb-P3) and 4 self-interactions (HC-Pro, VPg, NIaPro, and CP). These interactions did not show the same directionality as corresponding interactions detected in other potyviruses and consequently, a protein interaction matrix displayed different patterns. This initial map of the protein interactions of PRSV-P allows further study of various viral proteins in order to develop a new strategy to control PRSV-P infection.
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