A Novel Negative Fe-Deficiency-Responsive Element and a TGGCA-Type-Like FeRE Control the Expression of FTR1 in Chlamydomonas reinhardtii

文献类型： 外文期刊

第一作者： Fei, Xiaowen

作者： Fei, Xiaowen;Li, Yajun;Deng, Xiaodong;Fei, Xiaowen;Eriksson, Mats

作者机构：

期刊名称：JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY （ 影响因子：3.169； 五年影响因子：3.132 ）

ISSN： 1110-7243

年卷期： 2010 年

页码：

收录情况： SCI

摘要： We have reported three Fe-deficiency-responsive elements (FEREs), FOX1, ATX1, and FEA1, all of which are positive regulatory elements in response to iron deficiency in Chlamydomonas reinhardtii. Here we describe FTR1, another iron regulated gene and mutational analysis of its promoter. Our results reveal that the FeREs of FTR1 distinguish itself from other iron response elements by containing both negative and positive regulatory regions. In FTR1, the -291/-236 region from the transcriptional start site is necessary and sufficient for Fe-deficiency-inducible expression. This region contains two positive FeREs with a TGGCA-like core sequence: the FtrFeRE1 (ATGCAGGCT) at -287/-279 and the FtrFeRE2 (AAGCGATTGCCAGAGCGC) at -253/-236. Furthermore, we identified a novel FERE, FtrFeRE3 (AGTAACTGTTAAGCC) localized at -319/-292, which negatively influences the expression of FTR1.

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