An Fe Deficiency Responsive Element with a Core Sequence of TGGCA Regulates the Expression of FEA1 in Chlamydomonas reinharditii

文献类型： 外文期刊

第一作者： Fei, Xiaowen

作者： Fei, Xiaowen;Yang, Jinghao;Deng, Xiaodong;Fei, Xiaowen;Eriksson, Mats

作者机构：

关键词： Chlamydomonas reinharditii;FEA1;FeREs;iron;promoter

期刊名称：JOURNAL OF BIOCHEMISTRY （ 影响因子：3.387； 五年影响因子：3.169 ）

ISSN： 0021-924X

年卷期： 2009 年 146 卷 2 期

页码：

收录情况： SCI

摘要： Iron is essential to the unicellular green alga Chlamydomonas, but the molecular mechanism for response to iron deficiency remains largely unknown. In previous studies, we have identified FOX1 and ATX1 FEREs (Fe deficiency-responsive elements) as important regulation components of iron response in this organism. Here we present another iron regulated gene FEA1, which promoter was analysed by using a 5'- and 3'-end deletion and a scanning mutagenesis assay. The results reveal that the co-existence of -273/-188 and -118/-49 regions from transcriptional start site of FEA1 were sufficient and necessary for Fe deficiency-induced expression. Further deletion analysis indicates both -273/-253 and -103/-85 regions are essential for inducible expression. The scanning mutagenesis analysis of these regions identifies two cis-acting elements: the FeaFeRE1 at -273/-259 (CTGCGGTGGCAAAGT) and FeaFeRE2 at -106/-85 (CCGCCGCNNNTGGCACCAGCCT). Sequence comparison of FeaFeRE1 and FeaFeRE2 reveals a core sequence of TGGCA, which had been found in our previously reported Fe-deficiency-inducible gene ATX1. Moreover, we show that the promoter region of several genes, including FRE1, IRT1, ISCA, ZRT1, ZRT5, NRAMP2 and COPT1, also contains this core sequence, suggesting that at least two classes FeRE elements exist in Clamydomonas, one in FEA1 and ATX1 and others the second in FOX1, FEA2, MTP4, NRAMP3 and RBOL1.

分类号：

  • 相关文献

[1]The effects of copper, iron and zinc on digestive enzyme activity in the hybrid tilapia Oreochromis niloticus (L.) x Oreochromis aureus (Steindachner). Li, J. -S.,Li, J. -L.,Wu, T. -T.. 2007

[2]The effects of nutritional restriction on neutral lipid accumulation in Chlamydomonas and Chlorella. Deng, Xiaodong,Fei, Xiaowen,Li, Yajun,Fei, Xiaowen. 2011

[3]Titanium as a Beneficial Element for Crop Production. Lyu, Shiheng,Wei, Xiangying,Chen, Jianjun,Pan, Dongming,Lyu, Shiheng,Wei, Xiangying,Chen, Jianjun,Wang, Cun,Wang, Cun,Wang, Xiaoming. 2017

[4]Comparison on cellular mechanisms of iron and cadmium accumulation in rice: prospects for cultivating Fe-rich but Cd-free rice. Gao, Lei,Chang, Jiadong,Chen, Ruijie,Li, Hubo,Lu, Hongfei,Xiong, Jie,Gao, Lei,Chang, Jiadong,Chen, Ruijie,Li, Hubo,Lu, Hongfei,Xiong, Jie,Tao, Longxing. 2016

[5]Structure and expression of transferrin gene of channel catfish, Ictalurus punctatus. Liu, Hong,Takano, Tomokazu,Abernathy, Jason,Wang, Shaolin,Sha, Zhenxia,Jiang, Yanliang,Terhune, Jeffery,Kucuktas, Huseyin,Peatman, Eric,Liu, Zhanjiang,Liu, Hong,Takano, Tomokazu,Abernathy, Jason,Wang, Shaolin,Sha, Zhenxia,Jiang, Yanliang,Terhune, Jeffery,Kucuktas, Huseyin,Peatman, Eric,Liu, Zhanjiang,Liu, Hong,Sha, Zhenxia. 2010

[6]Combined treatment with vitamin A and iron to prevent piglet anemia. Jiang, JunFang,Jiang, YongQing,Jiang, JunBing,Zhu, HuiSen. 2009

[7]Determination of Dietary Iron Requirements by Full Expression of Iron-Containing Enzymes in Various Tissues of Broilers. Ma, Xinyan,Liao, Xiudong,Lu, Lin,Zhang, Liyang,Luo, Xugang,Ma, Xinyan,Li, Sufen.

[8]Effect of iron supplementation on intestinal function and oxidative stress in piglets with induced colitis. Chen, Q.,Le, G. W.,Shi, Y. H.,Zhang, Sh. M.,Jin, X..

[9]Identification and characterization of a Macrobrachium nipponense ferritin subunit regulated by iron ion and pathogen challenge. Sun, Shengming,Gu, Zhimin,Gu, Zhimin,Sun, Shengming,Fu, Hongtuo,Zhu, Jian,Ge, Xianping,Xuan, Fujun.

[10]Studies on resistance characteristic and cDNA sequence conservation of transferrin from crucian carp, Carassius auratus. Long, Hua,Yu, Qi-Xing.

[11]Characterization of natural variation for zinc, iron and manganese accumulation and zinc exposure response in Brassica rapa L.. Wu, Jian,Schat, Henk,Sun, Rifei,Koornneef, Maarten,Wang, Xiaowu,Aarts, Mark G. M..

[12]Effect of manganese spatial distribution in the soil profile on wheat growth in rice-wheat rotation. Liu, XJ,Li, L,Zhang, FS,Zeng, XZ,Tang, CX.

[13]Determination of dietary iron requirements by full expression of iron-containing cytochrome c oxidase in the heart of broilers from 22 to 42 d of age. Liao, Xiudong,Ma, Chunyan,Lu, Lin,Zhang, Liyang,Luo, Xugang. 2017

[14]Review: Breeding wheat for enhanced micronutrients. Xu, Yunfeng,An, Diaoguo,Xu, Hongxing,Li, Hongjie. 2011

[15]Can liming reduce cadmium (Cd) accumulation in rice (Oryza sativa) in slightly acidic soils? A contradictory dynamic equilibrium between Cd uptake capacity of roots and Cd immobilisation in soils. Yang, Yongjie,Chen, Jiangmin,Huang, Qina,Tang, Shaoqing,Hu, Peisong,Shao, Guosheng,Chen, Jiangmin,Wang, Jianlong. 2018

[16]Fouling of extracellular algal organic matter during ultrafiltration: The influence of iron and the fouling mechanism. Huang, Weiwei,Zhou, Wenzong,Lv, Weiguang,Huang, Weiwei,Hu, Mengliu,Qin, Xiao,Dong, Bingzhi. 2017

[17]Identification and characterization of the zinc-regulated transporters, iron-regulated transporter-like protein (ZIP) gene family in maize. Li, Suzhen,Huang, Yaqun,Zhu, Liying,Zhao, Yongfeng,Guo, Jinjie,Chen, Jingtang,Li, Suzhen,Zhou, Xiaojin,Zhang, Shaojun,Chen, Rumei. 2013

[18]Excessive nitrate enhances cadmium (Cd) uptake by up-regulating the expression of OsIRT1 in rice (Oryza sativa). Yang, Yongjie,Fu, Guanfu,Chen, Tingting,Tao, Longxing,Xiong, Jie,Chen, Ruijie,Xiong, Jie,Chen, Ruijie.

[19]Effect of wheat pearling on flour phytase activity, phytic acid, iron, and zinc content. Liu, Zhenghui,Wang, Haiyan,Wang, Xu-E,Xu, Hongyan,Chen, Peidu,Liu, Dajun,Gao, Derong,Zhang, Guoping. 2008

[20]Iron nutrition affects cadmium accumulation and toxicity in rice plants. Shao, Guosheng,Chen, Mingxue,Wang, Weixia,Mon, Renxiang,Zhang, Guoping.

作者其他论文 更多>>

微信小程序