Rapid one-step construction of hairpin RNA

文献类型： 外文期刊

第一作者： Yan, Pu

作者： Yan, Pu;Shen, Wentao;Gao, Xinzheng;Zhou, Peng;Duan, Jun

作者机构：

关键词： RNAi;hpRNA;OE-PCR;Asymmetric PCR;Plant;PRSV;Antivirus

期刊名称：BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS （ 影响因子：3.575； 五年影响因子：3.381 ）

ISSN： 0006-291X

年卷期： 2009 年 383 卷 4 期

页码：

收录情况： SCI

摘要： Hairpin RNA (hpRNA) is commonly used for gene-function exploration and gene engineering. In this study, a novel method was developed to construct intron-containing hairpin RNA (ihpRNA) rapidly and efficiently based on Overlap Extension PCR (OE-PCR). This method, Mixed One-step OE-PCR (MOOE-PCR), can amplify two inverted repeats of DNA fragments and a spliceable intron in parallel, and then assemble them to generate ihpRNA constructs in the same tube without the purification of intermediate products. This method required a PCR process of 38-40 cycles and ordinary PCR reagents. A total of 10 ihpRNA constructs were amplified successfully using this method, with the stems ranging from 50 bp to 484 bp in length, Our results suggest that this novel method is a useful strategy for constructing ihpRNA. (C) 2009 Elsevier Inc. All rights reserved.

分类号：

  • 相关文献

[1]Hairpin RNA Targeting Multiple Viral Genes Confers Strong Resistance to Rice Black-Streaked Dwarf Virus. Wang, Fangquan,Li, Wenqi,Zhu, Jinyan,Fan, Fangjun,Wang, Jun,Zhong, Weigong,Yang, Jie,Wang, Fangquan,Li, Wenqi,Zhu, Jinyan,Fan, Fangjun,Wang, Jun,Zhong, Weigong,Yang, Jie,Wang, Ming-Bo,Liu, Qing,Zhu, Qian-Hao,Zhou, Tong,Lan, Ying,Zhou, Yijun. 2016

[2]Comparison of factors that may affect the inhibitory efficacy of transgenic RNAi targeting of baculoviral genes in silkworm, Bombyx mori. Jiang, Liang,Zhao, Ping,Wang, Genhong,Cheng, Tingcai,Jin, Shengkai,Lin, Ping,Xia, Qingyou,Jiang, Liang,Zhao, Ping,Wang, Genhong,Cheng, Tingcai,Jin, Shengkai,Lin, Ping,Sun, Qiang,Xia, Qingyou,Yang, Qiong,Xiao, Yang.

[3]A novel simple extracellular leucine-rich repeat (eLRR) domain protein from rice (OsLRR1) enters the endosomal pathway and interacts with the hypersensitive-induced reaction protein 1 (OsHIR1). Zhou, Liang,Cheung, Ming-Yan,Zhang, Shi-Hong,Sun, Samuel Sai-Ming,Lam, Hon-Ming,Zhou, Liang,Cheung, Ming-Yan,Zhang, Shi-Hong,Sun, Samuel Sai-Ming,Lam, Hon-Ming,Zhang, Qi,Lei, Cai-Lin,Zhang, Shi-Hong.

[4]AN IMPROVED SITE-DIRECTED MUTAGENESIS PROCEDURE AND APPLICATION FOR IDENTIFICATION OF MUTANT CLONES. Yi, Ganjun.

[5]Assembling long heteroduplexes by asymmetric polymerase chain reaction and annealing the resulting single-stranded DNAs. Wang, Mugui,Wei, Chuchu,Ye, Xiufen,Liu, Jianping,Zhang, Cuicui,Chen, Hao,Zhang, Xiaobo,Tu, Jumin. 2015

[6]Mx Is Not Responsible for the Antiviral Activity of Interferon-alpha against Japanese Encephalitis Virus. Zhou, Jing,Wang, Shi-Qi,Zhang, Xiao-Min,Gao, Zhi-Can,Chen, Pu-Yan,Zhou, Bin,Wei, Jian-Chao,Liu, Ke,Ma, Zhi-Yong. 2017

[7]Obtained transgenic wheat expressing pac1 mediated by Agrobacterium is resistant against Barley yellow dwarf virus-GPV. Yan Fei,Zheng Yinying,Zhang Wenwei,Xiao Hong,Li Shifang,Cheng Zhuomin.

[8]Poly(I:C) inhibits porcine reproductive and respiratory syndrome virus replication in MARC-145 cells via activation of IFIT3. Zhang, Lili,Liu, Jie,Bai, Juan,Wang, Xiaoye,Liu, Xing,Jiang, Ping,Du, Yijun. 2013

[9]Development and Validation of a Multiplex Reverse Transcription PCR Assay for Simultaneous Detection of Three Papaya Viruses. Tuo, Decai,Shen, Wentao,Yang, Yong,Yan, Pu,Li, Xiaoying,Zhou, Peng,Tuo, Decai. 2014

[10]Generation of stable infectious clones of plant viruses by using Rhizobium radiobacter for both cloning and inoculation. Tuo, Decal,Fu, Lanlan,Shen, Wentao,Li, Xiaoying,Zhou, Peng,Yan, Pu. 2017

[11]NIa-Pro of Papaya ringspot virus interacts with Carica papaya eukaryotic translation initiation factor 3 subunit G (CpeIF3G). Gao, Le,Tuo, Decai,Shen, Wentao,Yan, Pu,Li, Xiaoying,Zhou, Peng,Gao, Le,Tuo, Decai,Shen, Wentao,Yan, Pu,Li, Xiaoying,Zhou, Peng,Gao, Le.

[12]NIa-pro of Papaya ringspot virus interacts with papaya methionine sulfoxide reductase B1. Gao, Le,Shen, Wentao,Yan, Pu,Tuo, Decai,Li, Xiaoying,Zhou, Peng,Gao, Le,Shen, Wentao,Yan, Pu,Tuo, Decai,Li, Xiaoying,Zhou, Peng,Gao, Le.

[13]A conserved unusual posttranscriptional processing mediated by short, direct repeated (SDR) sequences in plants. Tang, Wei,Liu, Yongsheng,Niu, Xiangli,Luo, Di,Gao, Shaopei,Chang, Lijuan,Zhou, Yuke,Luo, Xiaoli,Li, Yuxiang,Hou, Pei,Tang, Wei,Liu, Yongsheng,Niu, Xiangli,Luo, Di,Gao, Shaopei,Chang, Lijuan,Zhou, Yuke,Luo, Xiaoli,Li, Yuxiang,Hou, Pei,Tang, Wei,Liu, Yongsheng,Ren, Guangjun,Lu, Bao-Rong. 2010

[14]Interactions between nanoparticles and plants: phytotoxicity and defense mechanisms. Yang, Jie,Rui, Yukui,Cao, Weidong. 2017

[15]Extended biotic ligand model for predicting combined Cu-Zn toxicity to wheat (Triticum aestivum L.): Incorporating the effects of concentration ratio, major cations and pH. Wang, Xuedong,Ji, Dongxue,Chen, Xiaolin,Li, Xiaoxiu,Ma, Yibing,Ma, Yibing,Yang, Junxing.

[16]Cytoskeletal inhibitors suppress the stomatal opening of Vicia faba L. induced by fusicoccin and IAA. Huang, RF,Wang, XC,Lou, CH.

[17]Plant biotechnology in China. Huang, JK,Rozelle, S,Pray, C,Wang, QF.

[18]Bacillus ciccensis sp nov., isolated from maize (Zea mays L.) seeds. Liu, Yang,Li, Nannan,Zhang, Xin,Cao, Yanhua,Ge, Yuanyuan,Cheng, Chi,Liu, Yang,Zhao, Jiuran,Li, Nannan,Xiao, Ming,Eom, Mi Kyung,Kim, Song-Gun,Schumann, Peter.

[19]Cold-induced modulation and functional analyses of the DRE-binding transcription factor gene, GmDREB3, in soybean (Glycine max L.). Chen, Ming,Xu, Zhaoshi,Xia, Lanqin,Li, Liancheng,Dong, Jianhui,Wang, Qiaoyan,Ma, Youzhi,Cheng, Xianguo.

[20]RNAi-mediated plant protection against aphids. Yu, Xiu-Dao,Sun, Yong-Wei,Ma, You-Zhi,Xia, Lan-Qin,Yu, Xiu-Dao,Liu, Zong-Cai,Huang, Si-Liang,Chen, Zhi-Qin,Duan, Peng-Fei.

作者其他论文 更多>>

微信小程序