Isolation and characterization of microsatellites in snap bean

文献类型： 外文期刊

第一作者： Guo, JC

作者： Guo, JC;Hu, XW;Yanagihara, S;Yoshinobu, E

作者机构：

关键词： Phaseolus vulgaris;genomic library;microsatellite;molecular marker;DNA sequence analysis

期刊名称：ACTA BOTANICA SINICA （ 影响因子：0.599； ）

ISSN： 0577-7496

年卷期： 2000 年 42 卷 11 期

页码：

收录情况： SCI

摘要： The objectives of this study were to isolate and characterize microsatellites from a heat tolerant variety of snap bean (Phaseolus vulgaris L.) in order to generate polymorphic genetic markers linked to quantitative trait loci for heat tolerance. A genomic library contained 400 - 800 bp inserts was constructed and screened for the presence of (GA/CT)(n) and (CA/GT)(n) repeats. The proportion of positive clones yielded estimated of 3.72 x 10(4) such dinucleotide repeats per genome, roughly comparable to the abundance reported in other eukaryotic genomes. Twenty-six positive clones were sequenced. In contrast to mammalian genomes, the (GA/CT)(n) motif was much more abundant than the (CA/GT)(n) motif in these clones. The (GA/CT)(n) repeats also showed longer average repeat length (mean n = 10.4 versus 6.5), suggesting that they are better candidates for yielding polymorphic: genetic markers in the snap bean genome.

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