Screening of Vip3Aa60 and Vip3Ad5 and characterization of their binding to Spodoptera exigua midguts

文献类型： 外文期刊

第一作者： Pan, Zhi-Zhen

作者： Pan, Zhi-Zhen;Guan, Xiong;Xu, Lian;Zhang, Jing;Chen, Qing-Xi;Gao, Huan-Juan;Pan, Zhi-Zhen;Liu, Bo;Chen, Zheng;Zhu, Yu-Jing

作者机构：

关键词： Vip3Aa60;Vip3Ad5;Insecticidal activity;Spodoptera exigua;Bingding properties

期刊名称：PROCESS BIOCHEMISTRY （ 影响因子：3.757； 五年影响因子：3.665 ）

ISSN： 1359-5113

年卷期： 2017 年 61 卷

页码：

收录情况： SCI

摘要： Vip3A proteins, which shared no sequence or structural homology with the crystal proteins, were considered as a new generation of Bacillus thuringiensis toxins. In this study, we screened two novel vip3A genes from a number of B. thuringiensis strains, which were classified as vip3Aa60 and vip3Ad5 respectively. The full length of Vip3Aa60 and Vip3Ad5 showed 85% identity and their C-terminal domains showed 79.05% homology. The activity of both toxins was estimated against important lepidopteran pest (Spodoptera exigua). Vip3Aa60 revealed high toxicity against S.exigua as the LC50 value was 108.92 ng cm (-2) and Vip3Ad5 displayed low toxicity against S.exigua (LC50 > 4000 ng cm (-2)). In their binding assays, both Vip3Aa60 and Vip3Ad5 could bind to the Brush Border Membrane Vesicles (BBMV) of S. exigua and the affinity of binding to BBMV was significantly higher for Vip3Aa60 than Vip3Ad5. These results may provide useful information for insecticidal mechanism research of Vip3A against S. exigua.

分类号：

  • 相关文献

[1]Cloning and characterization of a novel Cry1A toxin from Bacillus thuringiensis with high toxicity to the Asian corn borer and other lepidopteran insects. Liang, Gemei,Li, Haitao,He, Kanglai,Song, Fuping,Feng, Xue,Zhang, Jie,Crickmore, Neil,Huang, Dafang.

[2]Bacillus thuringiensis insecticidal crystal proteins affect lifespan and reproductive performance of helicoverpa armigera and spodoptera exigua adults. YING ZHANG,YAN MA,PIN-JUN WAN,LI-LI MU,GUO-QING LI.

[3]Genetic effects on flight capacity in the beet armyworm, Spodoptera exigua (Lep., Noctuidae). Zhai, B. P.,Zhang, X. X.,Han, L. Z.,Gu, H. N.. 2009

[4]The Mechanism by Which Dodecyl Dimethyl Benzyl Ammonium Chloride Increased the Toxicity of Chlorpyrifos to Spodoptera exigua. Cui, Li,Yuan, Huizhu,Yang, Daibin,Rui, Changhui,Mu, Wei. 2017

[5]Vitellogenin and vitellogenin receptor gene expression profiles in Spodoptera exigua are related to host plant suitability. Zhao, Jing,Sun, Yang,Xiao, Liubin,Tan, Yongan,Jiang, Yiping,Bai, Lixin,Zhao, Jing. 2018

[6]Temporal allocation of metabolic tolerance in the body of beet armyworm in response to three gossypol-cotton cultivars. Wu Gang,Guo JianYing,Wan FangHao,Wu Gang,Harris, Marvin K.. 2009

[7]Mitochondria response to camptothecin and hydroxycamptothecine-induced apoptosis in Spodoptera exigua cells. Zhang, Lan,Zhang, Yanning,Mao, Liangang,Jiang, Hongyun. 2017

[8]Relationship of flight and reproduction in beet armyworm, Spodoptera exigua (Lepidoptera: Noctuidae), a migrant lacking the oogenesis-flight syndrome. Jiang, Xing Fu,Luo, Li Zhi,Sappington, Thomas W.. 2010

[9]Response of multiple generations of beet armyworm, Spodoptera exigua (Hubner), feeding on transgenic Bt cotton. Guo, J. -Y.,Wan, F. -H.,Harris, M. K.. 2009

[10]Establishment and characterization of three embryonic cell lines of beet armyworm, Spodoptera exigua (Lepidoptera: Noctuidae). Su, Rui,Zheng, Gui-Ling,Wan, Fang-Hao,Li, Chang-You,Wan, Fang-Hao.

[11]Combined effect of nucleopolyhedrovirus and Microplitis pallidipes for the control of the beet armyworm, Spodoptera exigua. Jiang, Jiexian,Ji, Xiangyun,Wan, Nianfeng,Chen, Xiaoqin,Zeng, Aiping.

[12]Effect of Temperature on Development, Survival, and Fecundity of Microplitis manilae (Hymenoptera: Braconidae). Xu, Zai-Fu,Qiu, Bo,Zhou, Zhong-Shi,Luo, Shu-Ping.

[13]New insights on the role of alkaline phosphatase 2 from Spodoptera exigua (Hubner) in the action mechanism of Bt toxin Cry2Aa. Zhao, Man,Wei, Jizhen,Zhang, Wanna,Wang, Bingjie,Khaing, Myint Myint,Liang, Gemei.

[14]EXPRESSION OF SNMP1 AND SNMP2 GENES IN ANTENNAL SENSILLA OF Spodoptera exigua (HUBNER). Liu, Chengcheng,Zhang, Jin,Dong, Shuanglin,Liu, Chengcheng,Zhang, Jin,Liu, Yang,Wang, Guirong.

[15]Identification and functional characterization of sex pheromone receptors in beet armyworm Spodoptera exigua (Hubner). Liu, Yang,Wang, Guirong,Liu, Chengcheng,Dong, Shuanglin,Walker, William B.. 2013

[16]Enhancement of attraction to sex pheromones of Spodoptera exigua by volatile compounds produced by host plants. Deng, JY,Wei, HY,Huang, YP,Du, JW. 2004

[17]Narrow tuning of an odorant receptor to plant volatiles in Spodoptera exigua (Hubner). Liu, Yang,Guo, Mengbo,Cao, Depan,Wang, Guirong,Liu, Chengcheng,Guo, Mengbo,Dong, Shuanglin. 2014

[18]A Fragment of Cadherin-Like Protein Enhances Bacillus thuringiensis Cry1B and Cry1C Toxicity to Spodoptera exigua (Lepidoptera: Noctuidae). Lu Qiong,Zhang Yong-jun,Cao Guang-chun,Zhang Li-li,Liang Ge-mei,Lu Yan-hui,Wu Kong-ming,Guo Yu-yuan,Lu Qiong,Zhang Li-li,Gao Xi-wu. 2012

[19]Brusatol isolated from Brucea javanica (L.) Merr. induces apoptotic death of insect cell lines. Feng, Xuehuan,Ma, Dejun,Yang, Jingjing,Jiang, Hongyun,Zhang, Yanning,He, Weizhi. 2013

[20]Enhancement of the biological activity of nucleopolyhedrovirus through disruption of the peritrophic matrix of insect larvae by chlorfluazuron. Fang, Ji-Chao,Liu, Bao-Sheng,Wang, Jie-Ping,Zhong, Wan-Fang,Wan, Fang-Hao.

作者其他论文 更多>>

微信小程序