Molecular cloning and expression analysis of mevalonate pyrophosphate decarboxylase in Antrodia cinnamomea

文献类型： 外文期刊

第一作者： Li, Jing

作者： Li, Jing;Shao, En-Si;Lin, Zhan-Xi;Lin, Xiong-Jie;Li, Jing;Shao, En-Si;Lin, Zhan-Xi

作者机构：

期刊名称：2016 2ND INTERNATIONAL CONFERENCE ON CHEMICAL MATERIALS AND PROCESS (ICCMP 2016)

ISSN： 2261-236X

年卷期： 2016 年 64 卷

页码：

收录情况： SCI

摘要： Mevalonate pyrophosphate decarboxylase (MVD) plays important roles in triterpenoid biosynthesis via the mevalonate pathway. A novel MVD gene was isolated and identified in Antrodia cinnamomea (Ac-mvd). The fulllength cDNA contained an open reading frame with a length of 1,209 bp and encoded a 402-amino acid polypeptide with a molecular mass of 43.3 kDa and a theoretical pI of 8.23. As incubation time was prolonged for 28 days, the triterpenoid content in the mycelium gradually increased and reached 39.192 +/- 2.025 mg/g; the triterpenoid content of the fruiting body grew in the hay of Cinnamomum kanehirae (ACFB-CK), was 49.391 +/- 2.675 mg/g, which was significantly different from other samples (P < 0.05). qRT-PCR revealed that the highest expression levels of Ac-mvd in the mycelium of Antrodia cinnamomea were detected on the 7th day. The expression levels gradually decreased as culture time was extended from 14 days to 42 days.

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