Expression of genes for two phosphofructokinases, tonoplast ATPase subunit A, and pyrophosphatase of tea roots in response to phosphorus-deficiency
文献类型: 外文期刊
第一作者: Lin, Zheng-He
作者: Lin, Zheng-He;Chen, Li-Song;Zhang, Fang-Zhou;Yang, Lin-Tong;Tang, Ning;Lin, Zheng-He;Chen, Rong-Bing
作者机构:
期刊名称:JOURNAL OF HORTICULTURAL SCIENCE & BIOTECHNOLOGY ( 影响因子:1.641; 五年影响因子:1.616 )
ISSN: 1462-0316
年卷期: 2010 年 85 卷 5 期
页码:
收录情况: SCI
摘要: Limited data are available on the levels of expression of the genes encoding ATP-dependent phosphofructokinase (ATP-PFK), pyrophosphate-dependent phosphofructokinase (PPi-PFK), tonoplast adenosinetriphosphatase (V-ATPase) subunit A, and tonoplast pyrophosphatase (V-PPiase) in response to phosphorus (P)-deficiency. Tea (Camellia sinensis) is a useful species in which to investigate adaptations to P-deficiency in higher plants due to its high tolerance to P-deficiency. Partial cDNAs encoding these four enzymes were isolated from tea roots. Thereafter, semi-quantitative RT-PCR was performed to analyse the levels of expression of these four genes in tea roots in response to P-deficiency. The results showed that P-deficiency decreased the levels of expression of the genes for ATP-PFK and V-ATPase subunit A, whereas not only did it not decrease, but it slightly increased the levels of expression of the genes for PPi-PFK and V-PPiase. This may contribute to the high tolerance of tea plants to P-deficiency.
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