Growth and pathogenicity characteristics of Ralstonia solanacearum strain RS1100 in long-term stationary phase culture

文献类型: 外文期刊

第一作者: Zhu, Y. -J.

作者: Zhu, Y. -J.;Xiao, R. -F.;Liu, B.

作者机构:

关键词: growth;pathogenicity;Ralstonia solanacearum;RS1100;stationary phase;viability

期刊名称:JOURNAL OF PLANT DISEASES AND PROTECTION ( 影响因子:1.928; 五年影响因子:1.652 )

ISSN: 1861-3829

年卷期: 2010 年 117 卷 4 期

页码:

收录情况: SCI

摘要: Nonsporulating gram-negative bacteria remain metabolically active and also develop increased resistance to a variety of environmental stresses after exponential growth has stopped and cells enter stationary phase. Ralstonia solanacearum is a nonsporulating gram-negative soil-borne pathogen that causes lethal wilt diseases of many plants around the world. Its growth and pathogenicity characteristics for entering long-term stationary phase were investigated by a prolonged 20-day laboratory culture. The data obtained from viability trial revealed that similar to 99% of R. solanacearum strain RS1100 (race 1) died at day 14 in SPA medium and then survivors maintained at similar to 10(5) cfu ml(-1) until the 20(th) day. Binary fission was observed and two modes of death procedures were assumed for growth of R. solanacearum under transmission electronic microscope, nucleoid condensation or nucleoid fragmentation and cytolysis. The latent period for R. solanacearum causing plant wilt prolonged while the cell density reduced gradually. The pathogen retained its aggressiveness at low density as 1.13 x 10(5) cfu ml(-1) at 20th day. Both mucoid and nonmucoid forms of R. solanacearum were coexistent throughout the experiment with varying ratio. The results here confirm that upon entering long-term stationary phase R. solanacearum can survive the stress conditions of nutrient starvation during prolonged stationary phase.

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