Long-Term Subculture and Maintenance of Transgenic Resistant Embryogenic Callus in Litchi (Litchi chinensis Sonn.)

文献类型： 外文期刊

第一作者： Lai, C. C.

作者： Lai, C. C.;Lai, Z. X.;Sang, Q. L.;Huang, Z. Y.;Huang, Q.;Lai, C. C.

作者机构：

关键词： litchi (Litchi chinensis Sonn.);transgenosis;resistant embryogenic callus;subculture and maintenance;GUS assay

期刊名称：III INTERNATIONAL SYMPOSIUM ON LONGAN, LYCHEE, AND OTHER FRUIT TREES IN SAPINDACEAE FAMILY

ISSN： 0567-7572

年卷期： 2010 年 863 卷

页码：

收录情况： SCI

摘要： The effects of subculture method, hygromycin, AgNO3, inoculation quantity and subculture cycle on the growth of litchi transgenic resistant embryogenic callus (TREC) were studied in this experiment. Meanwhile, the influences of the medium and long-term subculture on the GUS expression of litchi resistant embryogenic callus were carried out. The obtained results showed that litchi TREC could be steadily maintained for a long time when they were alternately subcultured in the MS medium containing 1.0 mg/L 2,4-D, 50 mg/L hygromycin and the MS medium supplemented with 1.0 mg/L 2,4-D, 100 mg/L inositol and 50 mg/L hygromycin.

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