Identification of self-incompatibility (S-) genotypes of Chinese apricot cultivars

文献类型： 外文期刊

第一作者： Zhang, Lijie

作者： Zhang, Lijie;Chen, Xuesen;Chen, Xiaoliu;Zhang, Chunyu;Liu, Xiaoli;Ci, Zhijuan;Zhang, Hong;Wu, Chuanjin;Liu, Chongqi;Zhang, Lijie

作者机构：

关键词： Prunus armeniaca;self-incompatibility;S-RNase;S-genotype

期刊名称：EUPHYTICA （ 影响因子：1.895； 五年影响因子：2.181 ）

ISSN： 0014-2336

年卷期： 2008 年 160 卷 2 期

页码：

收录情况： SCI

摘要： The S-genotypes of 16 apricot (Prunus armeniaca L.) cultivars native to China were determined by the S-allele PCR approach and the results were confirmed by cross-pollination tests among these cultivars. Primer combination EM-PC2consFD + EM-PC3consR, based on the conserved regions C2 and C3 of Rosaceous S-RNase genes, was the most useful primer combination for identifying Chinese apricot S-alleles. Twelve S-RNase alleles were identified using this primer combination, and they were defined as follows: S-9 was 657 bp, S-10 was 266 bp, S-11 was 464 bp, S-12 was 360 bp, S-13 was 401 bp, S-14 was 492 bp, S-15 was 469 bp, S-16 was 481 bp, S-17 was 487 bp, S-18 was 1337 bp, S-19 was 546 bp and S-20 was 1934 bp. S-11-S-20 were new S-RNase genes deposited in GenBank under accession numbers DQ868316, DQ870628-DQ870634, EF133689 and EF160078, respectively. Our findings contribute to a more efficient breeding program of Chinese apricot and further studies on the S-RNase genes.

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