Cloning and analysis of the Oswc-1 gene encoding a putative blue light photoreceptor from Ophiocordyceps sinensis

文献类型： 外文期刊

第一作者： Yang, Tao

作者： Yang, Tao;Dong, Caihong;Yang, Tao;Xiong, Weiping

作者机构：

关键词： Chinese caterpillar fungus;Light-inducible;Quantitative real-time PCR;White collar-1

期刊名称：MYCOSCIENCE （ 影响因子：1.223； 五年影响因子：1.266 ）

ISSN： 1340-3540

年卷期： 2014 年 55 卷 4 期

页码：

收录情况： SCI

摘要： Light plays an important role during both sexual and asexual growth of Ophiocordyceps sinensis, one of the best-known traditional Chinese medicines. In the present study, we cloned Oswc-1, the homologue of the blue light photoreceptor Ncwc-1 of Neurospora crassa, from O. sinensis by Hi-tail polymerase chain reaction. The deduced amino acid sequence of Oswc-1 contains the similar function domains as NcWC-1 including transcriptional activation, LOV (Light, Oxygen, or Voltage), PAS (Per-Arnt-Sim) and Zinc Finger domains. Phylogenetic analysis based on fungal WC-1 supported OsWC-1 was a blue light receptor. The expression of Oswc-1 messenger RNA was up-regulated upon irradiation significantly. (C) 2013 The Mycological Society of Japan. Published by Elsevier B. V. All rights reserved.

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