(Epi)catechin damage effects on the development of mouse intestinal epithelial structure through the PERK-eIF2 & alpha;-ATF4-CHOP pathway

文献类型： 外文期刊

第一作者： Guo, Shijie

作者： Guo, Shijie;Huang, Weiwei;Guo, Qingbin;Han, Jianzhong;Qin, Yumei;Han, Jianzhong;Qin, Yumei;Cao, Qingqing

作者机构：

期刊名称：FOOD & FUNCTION （ 影响因子：6.1； 五年影响因子：6.5 ）

ISSN： 2042-6496

年卷期： 2023 年 14 卷 14 期

页码：

收录情况： SCI

摘要： As powerful bioactive compounds found in a variety of plant-based foods, (epi)catechins have been identified to be associated with an abundant array of health benefits. While their adverse impacts have also been gaining increasing attention, their intestinal impact is still unclear. In this study, intestinal organoids were used as an in vitro model to analyze the effects of four (epi)catechins on the development of the intestinal epithelial structure. Morphological characteristics, oxidative stress, and endoplasmic reticulum (ER) stress assays with (epi)catechins treatment showed that (epi)catechins promoted intestinal epithelial apoptosis and stress response. These effects had dose-dependent and structural differences (EGCG > EGC > ECG > EC). Furthermore, GSK2606414, a protein kinase RNA (PKR)-like ER kinase (PERK) pathway inhibitor, confirmed that the PERK-eukaryotic translation initiation factor 2 & alpha; (eIF2 & alpha;)-activating transcription factor 4 (ATF4)-C/EBP-homologous protein (CHOP) pathway is closely related to the damage. In addition, the results for the intestinal inflammatory mouse model further verified that (epi)catechins significantly delayed intestinal repair. Taken together, these findings revealed that overdosage of (epi)catechins has damage potential on the intestinal epithelium and may increase the risk of intestinal damage.

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