Genetic and multi-omics analyses reveal BnaA07.PAP2(In-184-317) as the key gene conferring anthocyanin-based color in Brassica napus flowers

文献类型： 外文期刊

第一作者： Ye, Shenhua

作者： Ye, Shenhua;Ma, Tiantian;Ma, Xiaowei;Chen, Yanping;Wu, Lumei;Zhao, Lun;Yi, Bin;Ma, Chaozhi;Tu, Jinxing;Shen, Jinxiong;Fu, Tingdong;Wen, Jing;Hua, Shuijin

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关键词： Anthocyanins; Brassica napus; flower color; genetic transformation; insertions in the promoter region; map-based cloning; multi-omics; PAP2; RNA-seq

期刊名称：JOURNAL OF EXPERIMENTAL BOTANY （ 影响因子：7.298； 五年影响因子：8.291 ）

ISSN： 0022-0957

年卷期： 2022 年 73 卷 19 期

页码：

收录情况： SCI

摘要： Two insertions in the promoter region cause the transcriptional activation of BnaA07.PAP2, which launches the whole anthocyanin pathway to produce the newly apricot and pink colors in B. napusflowers. The molecular mechanisms underlying anthocyanin-based flower coloration remain unknown in Brassica napus. To identify the key genes and metabolites associated with apricot and pink flower colors, metabolome, BSA-seq, and RNA-seq analyses were conducted on apricot-, pink-, yellow-, and white-flowered F2B. napus. Yellow carotenoids and red anthocyanins were abundant in apricot petals, while colorless carotenoids and red anthocyanins accumulated in pink petals. Most carotenoid genes were not differentially regulated between apricot and yellow or between pink and white petals. Three regulator genes, BnaMYBL2, BnaA07.PAP2, and BnaTT8, and structural genes in anthocyanin biosynthesis were dramatically enhanced in apricot and pink petals in comparison with yellow and white petals. Map-based cloning revealed that BnaA07.PAP2 is responsible for anthocyanin-based flower color and encodes a nucleus-localized protein predominantly expressed in apricot and pink flowers. Two insertions in the promoter region are responsible for the transcriptional activation of BnaA07.PAP2 in flowers. Introducing the BnaA07.PAP2(In-184-317) allele broadly activated the expression of anthocyanin-related genes and promoted anthocyanin accumulation in flowers, yielding color change from yellow to apricot. These findings illustrate the genetic basis of anthocyanin-based flower coloration and provide a valuable genetic resource for breeding varieties with novel flower colors in B. napus.

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