cDNA Representational Difference Analysis of Deltamethrin-Resistant and -Susceptible Strains of Diamondback Moth

文献类型： 外文期刊

第一作者： Zhang, Hong

作者： Zhang, Hong;Cheng, Chen;Liu, Bai-Duo;Cheng, Luo-Gen;Li, Feng-Liang

作者机构：

关键词： cDNA RDA;insecticide resistance;Plutella xylostella;ribosomal protein L7;qRCR

期刊名称：PAKISTAN JOURNAL OF ZOOLOGY （ 影响因子：0.831； 五年影响因子：0.768 ）

ISSN： 0030-9923

年卷期： 2013 年 45 卷 2 期

页码：

收录情况： SCI

摘要： The diamondback moth (Plutella xylostella L.), a universal insect pest of cruciferous crops, has shown an extraordinary capacity to resist synthetic pesticides. Identification of genes associated with insecticide resistance has been a valuable tool to understand the mechanisms of resistance to insecticides. cDNA representational difference analysis (cDNA RDA) was performed to screen the upregulated genes in deltamethrin-resistant samples compared with the controls (susceptible strain). The fourth instar larve of deltamethrin-resistant and susceptible strains of diamondback moth were selected for this study. The cDNA of resistant strain was used as tester amplicon, the driver amplicon was from the susceptible strain. After three rounds of substractive hybridization, we obtained six differentially expressed genes. Comparison between our results and data in the GenBank showed that one differential sequence had high homology with ribosomal protein gene L7 (RpL7). To validate the result, RpL7 was cloned and RpL7 expression levels between the resistant samples and the controls were detected using real-time quantitative PCR (qPCR). The results showed that mRNA level of RpL7 was significantly higher in deltamethrin-resistant samples than in the controls. There are no direct reports indicating the upregulated gene RpL7 is associated with insecticide resistance, further studies are still needed.

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