ISOLATION AND CHARACTERIZATION OF A POLLEN-SPECIFIC GENE ZMSTK2_USP FROM ZEA MAYS

文献类型： 外文期刊

第一作者： Javeed, A.

作者： Javeed, A.;Fan, M. X.;Zhang, C. Y.;Ma, W. J.;Chen, M. M.;Liu, K. C.;Cai, F. C.;Lin, F.;Shi, L.;Hussain, K.

作者机构：

关键词： maize;serine/threonine kinase;pollen development;mutant;expression pattern;GUS activity

期刊名称：APPLIED ECOLOGY AND ENVIRONMENTAL RESEARCH （ 影响因子：0.711； 五年影响因子：0.796 ）

ISSN： 1589-1623

年卷期： 2018 年 16 卷 1 期

页码：

收录情况： SCI

摘要： Microsporogenesis in flowering plants is a complex process involving a range of gene expression. In the present study, a pollen-specific gene ZmSTK2_USP (serine threonine kinase) was isolated from Zea mays. Mutant ZmSTK2_USP showed 56.7% pollen viability capacity, 48.92% germination rates comparing with wild-type. Northern Blot results showed that ZmSTK2_USP were specifically expressed in mature pollen. The expression level was highest at late pollen development, followed by bicellular stage. And at early pollen stage, no expression was detected. GUS activity was detected in middle stage, late stage of pollens and germinated pollen. These results indicated that ZmSTK2_USP expressed at late pollen development and was pollen-specific.

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