Characterization of a TIR-NBS-LRR gene associated with downy mildew resistance in grape

文献类型： 外文期刊

第一作者： Fan, J. J.

作者： Fan, J. J.;Xu, X.;Ruan, Y. Y.;Zhu, Y. S.;Cui, Z. H.;Zhang, L. J.;Wang, P.;Liu, K.

作者机构：

关键词： Downy mildew;Nucleotide-binding site leucine-rich repeat;Plasmopara viticola;Rapid amplification of cDNA ends;Quantitative real-time polymerase chain reaction;Vitis spp

期刊名称：GENETICS AND MOLECULAR RESEARCH （ 影响因子：0.764； 五年影响因子：0.912 ）

ISSN： 1676-5680

年卷期： 2015 年 14 卷 3 期

页码：

收录情况： SCI

摘要： Grapevine downy mildew, caused by Plasmopara viticola, is a devastating disease that results in considerable economic losses as well as environmental damage through the repeated application of fungicides. The nucleotide-binding site leucine-rich repeat gene family functions in plant immunoactivity against various pathogens and pests. In this study, the 5' and 3' ends of the resistance gene homology fragment RGA5 were obtained by rapid amplification of cDNA ends. The 4282-base pair full-length cDNA was obtained using gene-specific primers, and the corresponding 1335-amino acid protein sequence contained characteristic nucleotide-binding site leucine- rich repeat domains of plant resistance proteins, including the toll-interleukin receptor type region. Expression of RGA5 during P. viticola infection and abiotic stress was investigated using quantitative real-time polymerase chain reaction. The results showed that treatment with P. viticola and 4 abiotic stimuli (salicyclic acid, methyl-jasmonate, abscisic acid, H2O2) significantly induced RGA5 within 12 days of inoculation. Therefore, RGA5 may play a critical role in protecting grapevines against P. viticola via signaling pathways involving these molecules.

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