A SCAR Marker for Detection Resistant Gene Ty-3 in Tomato

文献类型： 外文期刊

第一作者： Li Haitao

作者： Li Haitao;Liu Wenqi;Li Haitao;Zou Qingdao;Zhang Zijun

作者机构：

关键词： Tomato yellow leaf curl virus;Ty-3 gene;Molecular marker

期刊名称：THIRD CONFERENCE ON HORTICULTURE SCIENCE AND TECHNOLOGY (CHST 2012)

ISSN：

年卷期： 2012 年

页码：

收录情况： SCI

摘要： Tomato yellow leaf curl disease (TYLCD) is a new disease and causes important yield losses in tomato (Lycopersion esculentum Mill.) in China. For breeding resistant hybrids, PCR-based markers often were used for selection resistant materials. Most of the commercial cultivars resistant to TYLCD carry Ty-3 gene. In order to obtain molecular marker of resistant gene Ty-3, 3 homozygous resistant lines (Ty-3/Ty-3) and 3 homozygous susceptible lines (ty-3/ty-3) were used for PCR reaction. The result showed that homozygous resistant lines produced about 600bp fragment, and homozygous susceptible lines produced about 320bp fragment. So this marker could distinguish resistant and susceptible lines, and was a co-dominant marker tightly linked to Ty-3 gene. The marker was used to detect 24 important inbred lines and the result showed that all of them did not carry Ty-3 resistant gene. 23 individuals of F-2 segregation population from resistant hybrid were also be detected with this marker, and the result showed that 16 individuals carried Ty-3 resistant gene, including 4 homozygous resistant individuals. It's concluded that this marker is a useful SCAR marker for detecting Ty-3 resistant gene in tomato.

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