Cucumber Metallothionein-Like 2 (CsMTL2) Exhibits Metal-Binding Properties

文献类型： 外文期刊

第一作者： Pan, Yu

作者： Pan, Yu;Pan, Yanglu;Zhai, Junpeng;Li, Jinhua;Du, Xiaobing;Su, Chenggang;Zhang, Xingguo;Zhai, Junpeng;Xiong, Yan

作者机构：

关键词： cucumber;CsMTL2;metallothionein;metal-binding properties

期刊名称：GENES （ 影响因子：2.8； 五年影响因子：3.3 ）

ISSN： 2073-4425

年卷期： 2016 年 7 卷 12 期

页码：

收录情况： SCI

摘要： We identified a novel member of the metallothionein (MT) family, Cucumis sativus metallothionein-like 2 (CsMTL2), by screening a young cucumber fruit complementary DNA (cDNA) library. The CsMTL2 encodes a putative 77-amino acid Class II MT protein that contains two cysteine (Cys)-rich domains separated by a Cys-free spacer region. We found that CsMTL2 expression was regulated by metal stress and was specifically induced by Cd2+ treatment. We investigated the metal-binding characteristics of CsMTL2 and its possible role in the homeostasis and/or detoxification of metals by heterologous overexpression in Escherichia coli cells. Furthermore, we produced a deletion mutant form of the protein, CsMTL2m, that contained the two Cys-rich clusters but lacked the spacer region, in E. coli. We compared the metal-binding properties of CsMTL2 with those of CsMTL2m, the domain of human metallothionein-like protein 1 (HsMTXb), and phytochelatin-like (PCL) heterologously expressed in E. coli using metal-binding assays. We found that E. coli cells expressing CsMTL2 accumulated the highest levels of Zn2+ and Cd2+ of the four transformed cell types, with levels being significantly higher than those of control cells containing empty vector. E. coli cells expressing CsMTL2 had a higher tolerance for cadmium than for zinc ions. These findings show that CsMTL2 improves metal tolerance when heterologously expressed in E. coli. Future studies should examine whether CsMTL2 improves metal tolerance in planta.

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