Decolorization of Alizarin Red and other synthetic dyes by a recombinant laccase from Pichia pastoris

文献类型： 外文期刊

第一作者： Zheng, Miaomiao

作者： Zheng, Miaomiao;Shao, Shuli;Chi, Yujie;Yi, Hongwei

作者机构：

关键词： Alizarin Red;Neutral Red;Congo Red;Crystal Violet;Dye decolorization;Laccase;Pichia pastoris;Recombinant laccase

期刊名称：BIOTECHNOLOGY LETTERS （ 影响因子：2.0； 五年影响因子：2.4 ）

ISSN： 0141-5492

年卷期： 2014 年 36 卷 1 期

页码：

收录情况： SCI

摘要： A cDNA encoding for a laccase was isolated from the white-rot fungus Lenzites gibbosa by RT-PCR and expressed in the Pichia pastoris. The laccase native signal peptide efficiently directed the secretion of the recombinant laccase in an active form. Factors influencing laccase expression, such as pH, cultivation temperature, copper concentration and methanol concentration, were optimized. The recombinant enzyme was purified to electrophoretic homogeneity, and was estimated to have a MW of similar to 61.5 kDa. The purified enzyme behaved similarly to the native laccase produced by L. gibbosa and efficiently decolorized Alizarin Red, Neutral Red, Congo Red and Crystal Violet, without the addition of redox mediators. The decolorization capacity of this recombinant enzyme suggests that it could be a useful biocatalyst for the treatment of dye-containing effluents. This study is the first report on the synthetic dye decolorization by a recombinant L. gibbosa laccase.

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