THE DIFFERENTIAL GENE EXPRESSION OF KEY ENZYME IN THE GIBBERELLIN PATHWAY IN THE POTATO (SOLANUM TUBEROSUM) MUTANT M4P-9

文献类型: 外文期刊

第一作者: Shi, Jian-Bin

作者: Shi, Jian-Bin;Ye, Guang-Ji;Yang, Yong-Zhi;Wang, Fang;Zhou, Yun;Sun, Hai-Hong;Wang, Jian;Ye, Guang-Ji;Yang, Yong-Zhi;Wang, Fang;Zhou, Yun;Sun, Hai-Hong;Wang, Jian;Ye, Guang-Ji;Yang, Yong-Zhi;Wang, Fang;Zhou, Yun;Sun, Hai-Hong;Wang, Jian;Ye, Guang-Ji;Yang, Yong-Zhi;Wang, Fang;Zhou, Yun;Sun, Hai-Hong;Wang, Jian;Ye, Guang-Ji;Yang, Yong-Zhi;Wang, Fang;Zhou, Yun;Sun, Hai-Hong;Wang, Jian

作者机构:

关键词: Gene expression;Gibberellin pathway;Solanum tuberosum

期刊名称:PAKISTAN JOURNAL OF BOTANY ( 影响因子:0.972; 五年影响因子:0.988 )

ISSN: 0556-3321

年卷期: 2016 年 48 卷 3 期

页码:

收录情况: SCI

摘要: In the present study, the expression patterns of the key genes in the gibberellin synthesis pathway in the potato dwarf mutant M4P-9 were detected using quantitative real-time PCR. Using Actin as an internal control, CPS1, KS, KO, GA20ox1, and GA2ox1, genes for key gibberellin synthesis enzymes, were evaluated, along with a gibberellin receptor gene. The standard curves were obtained from dilutions of PCR product; the correlation coefficient for Actin was 0.995, and those for the target genes varied from 0.994 to 1.000. The expression patterns of gibberellin pathway genes in different growth stages and tissues were calculated according to the method of Pfaffl. These genes showed expression patterns that varied based on growth stage and tissue type. The higher expression levels of CPS1 and GA2ox1 in roots, the lower expression levels of GA20ox1 in roots during tuber formation stage; as well as the increased expression of GA20ox1 and GA2ox1 genes in stems during the tuber formation stage, likely play key roles in the plant height phenotype in M4P-9 mutant materials. This article provides a basis for researching the mechanism of gibberellin synthesis in potato.

分类号:

  • 相关文献

[1]Soft rot of potatoes caused by Bacillus amyloliquefaciens in Guangdong province, China. Wang, Li,Li, Xiao-Bo,Suo, Hai-Cui,An, Kang,Luo, Huan-Min,Liu, Xiao-Jin.

[2]Rapid Determination of Rice Cultivar Responses to the Sheath Blight Pathogen Rhizoctonia solani Using a Micro-Chamber Screening Method. Jia, Y.,Correa-Victoria, F.,McClung, A.,Zhu, L.,Liu, G.,Wamishe, Y.,Xie, J.,Marchetti, M. A.,Pinson, S. R. M.,Rutger, J. N.,Correll, J. C..

[3]Phenotypic and Genotypic Characterization of Phytophthora infestans Isolates from China. Li, Benjin,Chen, Qinghe,Lv, Xin,Lan, Chengzhong,Zhao, Jian,Qiu, Rongzhou,Weng, Qiyong.

[4]Overexpression of the Arabidopsis DREB1A gene enhances potato drought-resistance. Jia, X. X.,Qi, E. F.,Ma, S.,Hu, X. Y.,Wen, G. H.,Wang, Y. H.,Li, J. W.,Jia, X. X.,Qi, E. F.,Ma, S.,Hu, X. Y.,Wen, G. H.,Wang, Y. H.,Li, J. W.,Jia, X. X.,Qi, E. F.,Ma, S.,Hu, X. Y.,Wen, G. H.,Wang, Y. H.,Li, J. W.,Li, Y. T.,Zhang, X. H.,Wang, H. M.,Wang, W. T..

[5]Sporulation, Inoculation Methods and Pathogenicity of Ustilaginoidea albicans, the Cause of White Rice False Smut in China. Wang, Shu,Dong, Hai,Liu, Xiao-Zhou,Bai, Yuan-Jun,Yang, Hao,Li, Min,Li, Min.

[6]Genome-wide analysis of the Solanum tuberosum (potato) trehalose-6-phosphate synthase (TPS) gene family: evolution and differential expression during development and stress. Xu, Yingchun,Wang, Yanjie,Jin, Qijiang,Mattson, Neil,Yang, Liu. 2017

[7]Effect of Nitrogen Form on Gene Expression in Leaf Tissue of Greenhouse Grown Potatoes During Three Stages of Growth. Tai, Helen,De Koeyer, David,Li, Xiu-Qing,Luo, Sainan,Millard, Pete,Xiong, Xingyao. 2012

[8]Isolation and characterization of the organ-specific and light-inducible promoter of the gene encoding rubisco activase in potato (Solanum tuberosum). Qu, D.,Song, Y.,Li, W. M.,Pei, X. W.,Wang, Z. X.,Jia, S. R.,Zhang, Y. Q.. 2011

[9]Generation of double-virus-resistant marker-free transgenic potato plants. Bai, Yunfeng,Guo, Zhihua,Wang, Xiaoqi,Bai, Dongmei,Zhang, Weifeng. 2009

[10]Gene transcription analysis during interaction between potato and Ralstonia solanacearum. Li, G. C.,Jin, L. P.,Wang, X. W.,Xie, K. Y.,Huang, S. W.,Qu, D. Y.,Li, G. C.,Yang, Y.,van der Vossen, E. A. G.. 2010

[11]Differential gene expression as an indicator of nitrogen sufficiency in field-grown potato plants. Tai, Helen,De Koeyer, David,Li, Xiu-Qing,Luo, Sainan,Xiong, Xingyao,Millard, Pete. 2011

[12]DEVELOPMENT OF THREE-VITRIFICATION-BASED CRYOPRESERVATION PROCEDURES FOR SHOOT TIPS OF CHINA'S POTATO. Wang, Biao,Wang, Ren-rui,Li, Jing-wei,Ma, Yan-li,Wang, Qiao-chun,Wang, Ren-rui,Li, Min-fu,Sheng, Wan-min. 2013

[13]Removal of early fruiting branches impacts leaf senescence and yield by altering the sink/source ratio of field-grown cotton. Chen, Yizhen,Dong, Hezhong,Chen, Yizhen,Kong, Xiangqiang,Dong, Hezhong,Kong, Xiangqiang,Dong, Hezhong. 2018

[14]Transgenic expression of delta-6 and delta-15 fatty acid desaturases enhances omega-3 polyunsaturated fatty acid accumulation in Synechocystis sp PCC6803. Chen, Gao,Bian, Fei,Peng, Zhenying,Zhang, Yan,Yu, Jinhui,Xuan, Ning,Bi, Yuping,He, Qingfang,Chen, Gao,Bian, Fei,Peng, Zhenying,Zhang, Yan,Yu, Jinhui,Xuan, Ning,Bi, Yuping,He, Qingfang,He, Qingfang,Wang, Qiang,Qu, Shujie,Ge, Haitao. 2014

[15]Functional Expression of the Arachis hypogaea L. Acyl-ACP Thioesterases AhFatA and AhFatB Enhances Fatty Acid Production in Synechocystis sp PCC6803. Chen, Gao,Zhang, Yan,Peng, Zhenying,Fan, Zhongxue,Bian, Fei,Yu, Jinhui,Chen, Gao,Zhang, Yan,Peng, Zhenying,Fan, Zhongxue,Bian, Fei,Yu, Jinhui,Chen, Jun,Qin, Song,Chen, Jun,He, Qingfang. 2017

[16]Transcriptome Analysis of Sucrose Metabolism during Bulb Swelling and Development in Onion (Allium cepa L.). Zhang, Chunsha,Zhang, Hongwei,Liang, Yi,Zhan, Zongxiang,Liu, Bingjiang,Chen, Zhentai. 2016

[17]Analysis of Differentially Expressed Genes in Genic Male Sterility Cotton (Gossypium hirsutum L.) Using cDNA-AFLP. Xiaoding Ma,Chaozhu Xing,Liping Guo,Yangcang Gong,Hailin Wang,Yunlei Zhao,Jianyong Wu. 2007

[18]Exogenous nitric oxide delays salt-induced leaf senescence in cotton (Gossypium hirsutum L.). Kong, Xiangqiang,Wang, Tao,Li, Weijiang,Tang, Wei,Zhang, Dongmei,Dong, Hezhong,Wang, Tao,Dong, Hezhong.

[19]Variants and Gene Expression of the TLR2 Gene and Susceptibility to Mastitis in Cattle. Huang, Jinming,Liu, Li,Wang, Hongmei,Zhang, Cuixia,Ju, Zhihua,Wang, Changfa,Zhong, Jifeng.

[20]Genome-Wide Analysis of Gene Expression Provides New Insights into Cold Responses in Thellungiella salsuginea. Wang, Jiangshan,Cui, Feng,Hou, Lei,Zhao, Shuzhen,Xia, Han,Qiu, Jingjing,Li, Tingting,Zhang, Ye,Wang, Xingjun,Zhao, Chuanzhi,Wang, Jiangshan,Zhang, Quan,Qiu, Jingjing,Wang, Xingjun,Zhao, Chuanzhi. 2017

作者其他论文 更多>>