Interaction of MyoD and MyoG with Myoz2 gene in bovine myoblast differentiation

文献类型： 外文期刊

第一作者： Wei, Dawei

作者： Wei, Dawei;Song, Yaping;Jiang, Chao;Song, Xiaoyu;Wu, Hao;Zhang, Jiupan;Raza, Sayed Haidar Abbas;Alotaibi, Mashael Alhumaidi;Albiheyri, Raed;Al-Zahrani, Majid;Makhlof, Raafat T. M.;Alsaad, Mohammad A.;Makhlof, Raafat T. M.;Albiheyri, Raed;Abdelnour, Sameh A.;Quan, Guobo

作者机构：

关键词： Promoter; MyoD; MyoG; Myoz2 gene

期刊名称：RESEARCH IN VETERINARY SCIENCE （ 影响因子：2.554； 五年影响因子：2.564 ）

ISSN： 0034-5288

年卷期： 2022 年 152 卷

页码：

收录情况： SCI

摘要： This study aims to explore the functional role of Myoz2 in myoblast differentiation, and elucidate the potential factors interact with Myoz2 in promoter transcriptional regulation. The temporal-spatial expression results showed that the bovine Myoz2 gene was highest expressed in longissimus dorsi, and in individual growth stages and myoblast differentiation stages. Knockdown of Myoz2 inhibited the differentiation of myoblast, and negative effect of MyoD, MyoG, MyH and MEF2A expression on mRNA levels. Subsequently, the promoter region of bovine Myoz2 gene with 1.7 Kb sequence was extracted, and then it was set as eight series of deleted fragments, which were ligated into pGL3-basic to detect core promoter regions of Myoz2 gene in myoblasts and myotubes. Transcription factors MyoD and MyoG were identified as important cis-acting elements in the core promoter region (-159/+1). Also, it was highly conserved in different species based on dual-luciferase analysis and multiple sequence alignment analysis, respectively. Furthermore, a chromatin immunoprecipitation (ChIP) analysis combined with site-directed mutation and siRNA interference and overexpression confirmed that the combination of MyoD and MyoG occurred in region-159/+1, and played an important role in the regulation of bovine Myoz2 gene. These findings explored the regulatory network mechanism of Myoz2 gene during the development of bovine skeletal muscle.

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