Genetic diversity analysis of faba bean (Vicia faba L.) germplasms using sodium dodecyl sulfate-polyacrylamide gel electrophoresis

文献类型： 外文期刊

第一作者： Hou, W. W.

作者： Hou, W. W.;Shi, J. B.;Liu, Y. J.;Zhang, X. J.

作者机构：

关键词： Faba bean;Protein;SDS-PAGE;Genetic similarity;Cluster analysis

期刊名称：GENETICS AND MOLECULAR RESEARCH （ 影响因子：0.764； 五年影响因子：0.912 ）

ISSN： 1676-5680

年卷期： 2015 年 14 卷 4 期

页码：

收录情况： SCI

摘要： To investigate genetic diversity and relationships of 101 faba bean (Vicia faba L.), landraces and varieties from different provinces of China and abroad were analyzed by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE). A total of 2625 unambiguous and stable bands from 101 germplasms were detected, and 36 different bands were classified according to the electrophoretic mobility patterns of the proteins as determined by the SDS-PAGE analysis, of which 16 were polymorphic. Besides the common bands, the protein bands of 92, 75, 62, 40, 34, 17, and 13 kDa presented the highest frequencies of 92.08, 90.10, 99.01, 95.05, 95.05, 98.02, and 95.05%, respectively. The other 29 polymorphic protein bands showed higher polymorphism with 16.09 polymorphic bands in average. The genetic similarity of the 101 genotypes tested varied from 0.6111 to 0.9722, with an average of 0.7122. Cluster analysis divided the 101 genotypes into six major clusters, which was consistent with the systematic classification of faba bean done in previous studies. The overall results indicated that SDS-PAGE was a useful tool for genetic diversity analysis and laid a solid foundation for future faba bean breeding.

分类号：

  • 相关文献

[1]Assessment of genetic diversity by simple sequence repeat markers among forty elite varieties in the germplasm for malting barley breeding. Wang, Jun-mei,Yang, Jian-ming,Zhu, Jing-huan,Jia, Qiao-jun,Tao, Yue-zhi. 2010

[2]Study on the Change of Muscle Proteins During the Half-Dried Salt-Cured Silver Carp (Hypophthalmichthys molitrix) Processing. Gao, Ruichang,Yuan, Li,Su, Li,Huang, Xingyi,Yu, Gang,Li, Laihao.

[3]Impact of vacuum mixing on protein composition and secondary structure of noodle dough. Zhang, Yingquan,Wu, Liang,Kong, Yan,Wei, Yimin,Liu, Rui,Sun, Junmao,Xing, Yanan.

[4]Molecular cloning of type I collagen cDNA and nutritional regulation of type I collagen mRNA expression in grass carp. Yu, E. M.,Liu, B. H.,Wang, G. J.,Yu, D. G.,Xie, J.,Xia, Y.,Gong, W. B.,Wang, H. H.,Li, Z. F.,Wei, N..

[5]Characterization of insecticidal crystal protein cry gene of Bacillus thuringiensis from soil of Sichuan Basin, China and cloning of novel haplotypes cry gene. Zhu, Jun,Tang, Jie,Li, Yunyan,Zheng, Aiping,Li, Ping,Tan, Furong,Zheng, Aiping,Zhu, Jun,Tang, Jie,Li, Yunyan,Li, Ping.

[6]A MALDI-TOF based analysis of high molecular weight glutenin subunits for wheat breeding. Liu, Li,Wang, Aili,Ma, Junhong,Lan, Ping,Ma, Wujun,Liu, Li,Xia, Xianchun,He, Zhonghu,Wang, Aili,Yan, Yueming,He, Zhonghu,Bekes, Frank,Appels, Rudi. 2009

[7]Genetic diversity and relationship of global faba bean (Vicia faba L.) germplasm revealed by ISSR markers. Wang, Hai-fei,Zong, Xu-xiao,Guan, Jian-ping,Yang, Tao,Sun, Xue-lian,Ma, Yu,Redden, Robert. 2012

[8]Allelic variation at the Glu-1 and Glu-3 loci, presence of the 1B.1R translocation, and their effects on mixographic properties in Chinese bread wheats. Liu, L,He, ZH,Yan, J,Zhang, Y,Xia, XC,Pena, RJ. 2005

[9]Identification and functional analysis of a new glyphosate resistance gene from a fungus cDNA library. Tao, Bo,Shao, Bai-Hui,Qiao, Yu-Xin,Wang, Xiao-Qin,Chang, Shu-Jun,Qiu, Li-Juan. 2017

[10]Diversity maintenance and use of Vicia faba L. genetic resources. Duc, Gerard,Bao, Shiying,Baum, Michael,Redden, Bob,Sadiki, Mohammed,Jose Suso, Maria,Vishniakova, Margarita,Zong, Xuxiao. 2010

[11]Comparative proteome analysis of glutenin synthesis and accumulation in developing grains between superior and poor quality bread wheat cultivars. Ma, Wujun,Liu, Wan,Gao, Xuan,Wang, Ke,Wang, Shunli,Yan, Yueming,Zhang, Yanzhen,Zhang, Yong,He, Zhonghu,Zhang, Yong,He, Zhonghu. 2012

[12]Genetic linkage map of Chinese native variety faba bean (Vicia faba L.) based on simple sequence repeat markers. Ma, Yu,Yang, Tao,Guan, Jian-Ping,Sun, Xue-Lian,Jiang, Jun-Ye,Gong, Cui-Xiang,Zong, Xu-Xiao,Bao, Shi-Ying,He, Yu-Hua,Hu, Jin-Guo. 2013

[13]Identification of SNPs and development of allelic specific PCR markers for high molecular weight glutenin subunit D(t)x1.5 from Aegilops tauschii through sequence characterization. Yang, WY,Zhang, WJ,Lu, BR. 2005

[14]Drought stress impact on leaf proteome variations of faba bean (Vicia faba L.) in the Qinghai-Tibet Plateau of China. Li, Ping,Wu, Xuexia,Liu, Yujiao,Li, Ping,Liu, Yujiao,Zhang, Yanxia,Li, Ping,Liu, Yujiao. 2018

[15]Structure and function of seed storage proteins in faba bean (Vicia faba L.). Liu, Yujiao,Wu, Xuexia,Hou, Wanwei,Li, Ping,Sha, Weichao,Tian, Yingying. 2017

[16]Effects of intercropping and nitrogen application on nitrate present in the profile of an Orthic Anthrosol in Northwest China. Li, WX,Li, L,Sun, JH,Guo, TW,Zhang, FS,Bao, XG,Peng, A,Tang, C. 2005

[17]Genetic Diversity Analysis of Faba Bean (Vicia faba L.) Based on EST-SSR Markers. Gong Ya-ming,Xu Sheng-chun,Hu Qi-zan,Zhang Gu-wen,Ding Ju,Mao Wei-hua,Li Ze-yun. 2011

[18]Effects of nitrogen and phosphorus fertilizers and intercropping on uptake of nitrogen and phosphorus by wheat, maize, and faba bean. Li, WX,Li, L,Sun, JH,Zhang, FS,Christie, P. 2003

[19]Analysis of ginsenoside content, functional genes involved in ginsenosides biosynthesis, and activities of antioxidant enzymes in Panax quinquefolium L. adventitious roots by fungal elicitors. Li, Jinxin,Li, Hongfa,Wang, Juan,Liu, Shujie,Li, Jianli,Liu, Dahui.

[20]Genetic diversity and phylogenetic relationship of Tadehagi in southwest China evaluated by inter-simple sequence repeat (ISSR). He, Xin,Huang, Bizhi,Liu, Diqiu,Liu, Guodao.

作者其他论文 更多>>

微信小程序