文献类型： 外文期刊

第一作者： Yin Cheng-Ming

作者： Yin Cheng-Ming;Ren Li-Chao;Lan Jiang-Feng;Wang Zhe;Chai Lian-Qin;Li Ning-Qiu

作者机构：

关键词： Lysozyme; White spot syndrome virus; Procambarus clarkii; Antimicrobial peptides

期刊名称：FISH AND SHELLFISH IMMUNOLOGY REPORTS

ISSN： 2667-0119

年卷期： 2021 年 2 卷

页码：

收录情况： SCI

摘要： Lysozyme is an important immune effector in innate immunity against pathogen infection. But the study on the active region of lysozyme is limited. In this study, two highly homologous lysozymes were identified from crayfish (designated as PcLysi4 and PcLysi5). The molecular structures of PcLysi4 and PcLysi5 were predicted by SWISS-MODEL with the structure of lysozyme (PDB accession No. 4PJ2.2.B) as model. The results suggested that the structure of PcLysi4 and PcLysi5 were highly similar, but there were more a-helices at positions (127-139) and longer beta-sheet at positions (49-57) in the structure of PcLysi5 than in that of PcLysi4. The antibacterial and antiviral functions of the two lysozymes were investigated. PcLysi4 and PcLysi5 could promote the bacterial clearance ability of crayfish, and increase the survival rate of Vibrio-infected crayfish. Further study showed that PcLysi5 inhibited WSSV replication, and enhanced the survival rate of WSSV-infected crayfish. There was no evidence that PcLysi4 has an influence on WSSV replication. Furthermore, PcLysi5 was detected to interact with envelope protein VP24 of WSSV. Our results would provide a new reference for the study on active region of lysozyme.

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