RNA-seq based gene expression analysis of ovarian granulosa cells exposed to zearalenone in vitro: significance to steroidogenesis

文献类型： 外文期刊

第一作者： Zhang, Guo-Liang

作者： Zhang, Guo-Liang;Zhang, Rui-Qian;Sun, Xiao-Feng;Cheng, Shun-Feng;Wang, Yu-Feng;Ge, Wei;Zhao, Yong;Shen, Wei;Li, Lan;Zhang, Guo-Liang;Ji, Chuan-Liang;Yu, Jie;Zhang, Guo-Liang;Sun, Shi-Duo;Feng, Yan-Zhong

作者机构：

关键词： porcine;granulosa cells;steroidogenesis;gene expression;RNA-seq

期刊名称：ONCOTARGET （ 影响因子：5.168； 五年影响因子：5.312 ）

ISSN： 1949-2553

年卷期： 2017 年 8 卷 38 期

页码：

收录情况： SCI

摘要： Zearalenone (ZEA) is a natural contaminant of various food and feed products representing a significant problem worldwide. Since the occurrence of ZEA in grains and feeds is frequent, the present study was carried out to evaluate the possible effects of ZEA on steroid production and gene expression of porcine granulosa cells, using RNA-seq analysis. Porcine granulosa cells were administered 10 mu M and 30 mu M ZEA during 72 h of culture in vitro. Following ZEA treatment the gene expression profile of control and exposed granulosa cells was compared using RNA-seq analysis. The results showed that in the exposed granulosa cells ZEA significantly altered the transcript levels, particularly steroidogenesis associated genes. Compared with the control group, 10 mu M and 30 mu M ZEA treatment significantly increased the mRNA expression of EDN1, IER3, TGF beta and BDNF genes and significantly reduced the mRNA expression of IGF-1 and SFRP2 genes. In particular, ZEA significantly decreased the expression of genes essential for estrogen synthesis including FSHR, CYP19A1 and HSD17 beta in granulosa cells. Furthermore, Q-PCR and Western-blot analysis also confirmed reduced expression of these genes in ZEA exposed granulosa cells. These effects were associated with a significant reduction of 17 beta-estradiol concentrations in the culture medium of granulosa cells. Collectively, these results demonstrated a concretely deleterious effect of ZEA exposure on the mRNA expression of steroidogenesis related genes and the production of steroid hormones in porcine ovarian granulosa cells in vitro.

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