Expression Profiles of the Heat Shock Protein 70 Gene in Response to Heat Stress in Agrotis c-nigrum (Lepidoptera: Noctuidae)

文献类型： 外文期刊

第一作者： Wang, Ling

作者： Wang, Ling;Yang, Shuai;Zhao, Kuijun;Han, Lanlan;Yang, Shuai

作者机构：

关键词： heat shock protein 70;Agrotis c-nigrum Linnaeus;expression profile;heat stress

期刊名称：JOURNAL OF INSECT SCIENCE （ 影响因子：1.857； 五年影响因子：1.904 ）

ISSN： 1536-2442

年卷期： 2015 年 15 卷

页码：

收录情况： SCI

摘要： Heat shock proteins (HSPs) are molecular chaperones, and their overexpression enhances the survivability and stress tolerance of the cell. To understand the characteristics of HSP70 in Agrotis c-nigrum Linnaeus larvae, the coding sequence of this protein was cloned, and the effect of heat stress on transcription and protein properties was assessed. The obtained cDNA sequence of HSP70 was 2,213 bp, which contained an ORF of 1,965 bp and encoded 654 amino acid residues. Isolated HSP70 cDNA demonstrated more than 80% identity with the sequences of other known insect HSP70s. Next, HSP70 was expressed in Escherichia coli BL21 (DE3) cells and identified using SDS-PAGE and western blotting analyses. In addition, anti-HSP70-specific antisera were prepared using a recombinant HSP70 protein, and the results showed that this antisera was very specific to AcHSP70. Real-time quantitative polymerase chain reaction detected the relative transcription of the HSP70 gene in larvae and the transcription of A. c-nigrum in response to high temperatures. Induction of HSP70 was up-regulated to peak expression at 36 degrees C.

分类号：

  • 相关文献

[1]Overexpression of heat shock protein 70 and its relationship to intestine under acute heat stress in broilers: 2. Intestinal oxidative stress. Gu, X. H.,Hao, Y.,Wang, X. L.. 2012

[2]Genome-wide analysis of the CaHsp20 gene family in pepper: comprehensive sequence and expression profile analysis under heat stress. Guo, Meng,Liu, Jin-Hong,Lu, Jin-Ping,Zhai, Yu-Fei,Wang, Hu,Gong, Zhen-Hui,Lu, Ming-Hui,Wang, Shu-Bin. 2015

[3]NPR1-dependent salicylic acid signaling is not involved in elevated CO2-induced heat stress tolerance in Arabidopsis thaliana. Li, Xin,Ahammed, Golam Jalal,Li, Xin,Yu, Jingquan,Shi, Kai. 2015

[4]Expression of heat shock protein 70 in transport-stressed broiler pectoralis major muscle and its relationship with meat quality. Xing, T.,Han, M. Y.,Xu, X. L.,Zhou, G. H.,Wang, M. F.,Zhu, X. S.. 2017

[5]Transcript analysis and expression profiling of three heat shock protein 70 genes in the ectoparasitoid Habrobracon hebetor (Hymenoptera: Braconidae). Chen, Hao-Liang,Zhangl, Hong-Yu,Chen, Hao-Liang,Chen, Hao-Liang,Throne, James E.,Chen, Hao-Liang,Zhu, Kun Yan. 2014

[6]Genomic, evolutionary and expression profile analysis of Hsp70 superfamily in A and D genome of cotton (Gossypium spp.) under the challenge of Verticillium dahliae. Xiao, Songhua,Yu, Jingzhong,Yu, Deyue,Xiao, Songhua,Xu, Jianwen,Zhao, Jun,Liu, Jianguang,Wu, Qiaojuan. 2017

[7]Effects of pathogenic bacterial challenge after acute sublethal ammonia-N exposure on heat shock protein 70 expression in Botia reevesae. Qi, Zemin,Zou, Yuanchao,Yue, Xingjian,Xie, Biwen,Zhao, Daxian,Gong, Quan.

[8]SjHSP70, a recombinant Schistosoma japonicum heat shock protein 70, is immunostimulatory and induces protective immunity against cercarial challenge in mice. Duan, Ming Ming,Xu, Rui Min,Yuan, Chun Xiu,Li, Yun Yan,Liu, Qun,Cheng, Guo Feng,Lin, Jiao-Jiao,Feng, Xin Gang,Li, Yun Yan,Liu, Qun,Lin, Jiao-Jiao.

[9]AtFes1A is Essential for Highly Efficient Molecular Chaperone Function in Arabidopsis. Fu, Can,Liu, Xiaxia,Yang, Wenwen,Yu, Hongbin,Liu, Jian,Zhang, Jingxia.

[10]Eimeria tenella heat shock protein 70 enhances protection of recombinant microneme protein MIC2 subunit antigen vaccination against E. tenella challenge. Zhang, Lei,Ma, Liping,Liu, Renqiang,Zhang, Yunfei,Zhang, Shouping,Hu, Chunmei,Song, Meng,Wang, Ming,Cai, Jianping. 2012

[11]Overexpression of heat shock protein 70 and its relationship to intestine under acute heat stress in broilers: 1. Intestinal structure and digestive function. Hao, Y.,Gu, X. H.,Wang, X. L.. 2012

[12]Alteration of HSF3 and HSP70 mRNA expression in the tissues of two chicken breeds during acute heat stress. Zhang, W. W.,Kong, L. N.,Zhang, X. Q.,Luo, Q. B.,Zhang, W. W.,Kong, L. N.,Zhang, X. Q.,Luo, Q. B.,Zhang, W. W.,Kong, L. N.,Zhang, X. Q.,Luo, Q. B.. 2014

[13]Molecular characterization of an ethephon-induced Hsp70 involved in high and low-temperature responses in Hevea brasiliensis. Zhang, Zhi-Li,Zhu, Jia-Hong,Zhang, Quan-Q I.,Cai, Yuan-Bao,Zhang, Zhi-Li.

[14]Deficient and excess dietary selenium levels affect growth performance, blood cells apoptosis and liver HSP70 expression in juvenile yellow catfish Pelteobagrus fulvidraco. Hu, Jun-Ru,Wu, Ying-Xia,Xian, Jian-An,Wang, An-Li,Hu, Jun-Ru,Huang, Yan-Hua,Wang, Guo-Xia,Cao, Jun-Ming.

[15]Differential expression of heat shock protein 90, 70, 60 in chicken muscles postmortem and its relationship with meat quality. Zhang, Muhan,Wang, Daoying,Geng, Zhiming,Sun, Chong,Bian, Huan,Xu, Weimin,Zhu, Yongzhi,Li, Pengpeng,Xu, Weimin.

[16]Effects of high temperature on life history traits and heat shock protein expression in chlorpyrifos-resistant Laodelphax striatella. Wang, Lihua,Shan, Dan,Zhang, Yueliang,Sun, Yang,Zhang, Zhichun,Fang, Jichao,Shan, Dan,Liu, Xiangdong.

[17]Genome-wide analysis of the WRKY gene family in cotton. Dou, Lingling,Zhang, Xiaohong,Pang, Chaoyou,Song, Meizhen,Wei, Hengling,Fan, Shuli,Yu, Shuxun.

[18]Heat shock factors in tomatoes: genome-wide identification, phylogenetic analysis and expression profiling under development and heat stress. Yang, Xuedong,Zhu, Weimin,Zhang, Hui,Liu, Na,Tian, Shoubo. 2016

[19]Genome-wide identification and characterization of GRAS transcription factors in sacred lotus (Nelumbo nucifera). Wang, Yu,Shi, Shenglu,Zhou, Ying,Zhou, Yu,Tang, Xiaoqing,Yang, Jie. 2016

[20]Molecular characterization of Pod1 during sex development in Chinese tongue sole (Cynoglossus semilaevis). Wang, Linna,Zhu, Ying,Xu, Wenteng,Shao, Changwei,Dong, Zhongdian,Li, Hailong,Cui, Zhongkai,Meng, Liang,Guo, Hua,Tian, Yongsheng,Chen, Songlin,Wang, Linna,Xu, Wenteng,Shao, Changwei,Tian, Yongsheng,Chen, Songlin. 2017

作者其他论文 更多>>

微信小程序