Cryoprotectants Optimization for Kazachstania Unispora Y15
文献类型: 外文期刊
第一作者: Li, Meng
作者: Li, Meng;Shao, Shushuang;Tao, Yang;Fu, Haiyan;Hong, Lei;Li, Xiuliang;Han, Dequan;Ping, Wenxiang;Feng, Liu
作者机构:
关键词: Kazachstania unispora;cryoprotectant;orthogonal experiment;optimization
期刊名称:2015 4TH INTERNATIONAL CONFERENCE ON ENERGY AND ENVIRONMENTAL PROTECTION (ICEEP 2015)
ISSN:
年卷期: 2015 年
页码:
收录情况: SCI
摘要: Cryoprotectants were selected and optimized to reduce the damage in freeze-drying process to Kazachstania unispora Y15 through single-factor experiment and orthogonal experiment. Evaluated by the survival rate of cells, cryoprotectants and the amount used were determined through single-factor experiment. The optimum formulation of cryoprotectant was gotten in orthogonal experiment: sucrose 22g/100mL, skim milk 15g/100mL, polyethylene glycol-1000 1.1g/100mL, L-glutamic acid sodium 7g/100mL, Kazachstania unispora Y15 freeze-dried survival rate was 94.11% with the optimum cryoprotectants.
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