Agrobacterium-mediated transformation of tomato with the ICE1 transcription factor gene

文献类型: 外文期刊

第一作者: Juan, J. X.

作者: Juan, J. X.;Yu, X. H.;Jiang, X. M.;Li, W.;Juan, J. X.;Yu, X. H.;Jiang, X. M.;Li, W.;Gao, Z.;Zhang, Y.;Duan, Y. D.;Yang, G.

作者机构:

关键词: Cold transcription factor;ICE1 gene;Tomato;Plant expression vector

期刊名称:GENETICS AND MOLECULAR RESEARCH ( 影响因子:0.764; 五年影响因子:0.912 )

ISSN: 1676-5680

年卷期: 2015 年 14 卷 1 期

页码:

收录情况: SCI

摘要: ICE1 genes play a very important role in plants in cold conditions. To improve the cold resistance of tomato, the ICE1 gene of Arabidopsis thaliana was used to construct the plant expression vector p3301-ICE1, and was overexpressed in tomato through Agrobacterium-mediated transformation. Five strains of resistant plants were obtained. PCR and half-quantitative results showed that the ICE1 gene was transferred to tomato; three strains tested positive. After low-temperature stress treatment, praline content and peroxide and catalase activities in the transgenic tomato plants were higher compared with non-transgenic controls, while malondialdehyde content was clearly lower.

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