EFFECTS OF MECHANICAL DELIPATION IN PORCINE OOCYTES ON MITOCHONDRIAL DISTRIBUTION, ROS ACTIVITY AND VIABILITY AFTER VITRIFICATION

文献类型： 外文期刊

第一作者： Ren, Liang

作者： Ren, Liang;Liu, Di;Fu, Bo;Fu, Bo;Fu, Bo;Ma, Hong

作者机构：

关键词： mitochondrial distribution;reactive oxygen species;porcine oocyte;vitrification

期刊名称：CRYOLETTERS （ 影响因子：1.066； 五年影响因子：1.0 ）

ISSN： 0143-2044

年卷期： 2015 年 36 卷 1 期

页码：

收录情况： SCI

摘要： BACKGROUND: Porcine oocytes were sensitive to cooling because of the presence of excessive lipids. OBJECTIVE: To evaluate the effect of lipid removal on. the mitochondrial distribution, the formation of reactive oxygen species (ROS) and the viability of porcine oocytes after vitrification. MATERIALS AND METHODS: Porcine oocytes were cultured in vitro, and lipid droplets were removed at the ME stage by mechanical delipation via micromanipulation. Mitochondrial distribution, ROS activity and oocyte viability were assessed after delipation and vitrification. RESULTS: Vitrification disrupted mitochondrial distribution in oocytes. The vitrified groups had a significantly lower rate of oocytes with normal mitrochondrial distribution than the fresh control group (39.6% and 58.5% versus 88.9%). The percentage of oocytes with normal mitochondrial distribution was significantly lower in the delipated group than that in the undelipated group after vitrification (39.6% vs 58.5%, p < 0.05). Vitrification also increased the ROS activity (p < 0.05); but there was no significant difference between the delipated and the undelipated groups (p > Delipated oocytes developed into blastocysts via parthenogenetic activation without vitrification. Delipation significantly decreased the rate of blastocyst formation. Vitrification also decreased the rates of cleavage and blastocyst formation (p < 0.05). The delipated group had a significantly higher cleavage rate than the undelipated group after vitrification (21.4% versus 10.4%). CONCLUSION: Lipid removal at the MU stage via micromanipulation impaired their subsequent development of porcine oocytes. Although vitrification causes an abnormal distribution of mitochondria and an increase in ROS production in porcine oocytes, the removal of lipid droplets improves subsequent development after vitrification.

分类号：

  • 相关文献

[1]Effect of vitrification on meiotic maturation, mitochondrial distribution and glutathione synthesis in immature silver fox cumulus oocyte complexes. Cao, Xinyan,Xue, Hailong,Wang, Shiyong,Zhao, Weigang,Du, Zhanyu,Yang, Yifeng,Yue, Zhigang,Cao, Xinyan,Xue, Hailong,Wang, Shiyong,Zhao, Weigang,Du, Zhanyu,Yang, Yifeng,Yue, Zhigang,Li, Jingchun.

[2]The Effects of Polyadenylation Status on MPFs During In Vitro Porcine Oocyte Maturation. Liu, Huiyu,Gao, Yan,Jiang, Hao,Ding, Yu,Deng, Qiong,Yu, Xianfeng,Zhang, Jiabao,Zhai, Bo,Zhang, Lianjiang,Li, Changhong,Zhang, Jiabao. 2016

[3]Effect of Vitrification on Mitochondrial Distribution and Membrane Potential in Mouse Two Pronuclear (2-PN) Embryos. Zhao, Xue-Ming,Fu, Xiang-Wei,Hou, Yun-Peng,Yan, Chang-Liang,Suo, Lun,Wang, Yan-Ping,Zhu, Shi-En,Zhao, Xue-Ming,Fu, Xiang-Wei,Hou, Yun-Peng,Yan, Chang-Liang,Suo, Lun,Wang, Yan-Ping,Zhu, Shi-En,Zhao, Xue-Ming,Zhu, Hua-Bin,Dinnyes, Andras.

[4]Developmental Potential of Vitrified Holstein Cattle Embryos Fertilized In Vitro with Sex-Sorted Sperm. Xu, J,Guo, Z,Su, L,Nedambale, TL,Zhang, J,Schenk, J,Moreno, JF,Dinnyes, A,Ji, W,Tian, XC,Yang, X,Du, F.

[5]Effects of cryopreservation on the survival rate of the seven-band grouper (Epinephelus septemfasciatus) embryos. Tian, Yongsheng,Jiang, Jing,Song, Lini,Chen, Zhangfan,Wang, Na,Chen, Songlin,Jiang, Jing,Song, Lini,Zhai, Jieming,Liu, Jiangchun.

[6]Optimization of cryopreservation of buffalo (Bubalus bubalis) blastocysts produced by in vitro fertilization and somatic cell nuclear transfer. Yang, C. Y.,Pang, C. Y.,Yang, B. Z.,Li, R. C.,Liang, X. W.,Lu, Y. Q..

[7]Vitrification-cryopreservation, an efficient method for eliminating Candidatus Liberobacter asiaticus, the citrus Huanglongbing pathogen, from in vitro adult shoot tips. Ding, Fang,Zhong, Yun,Cao, Qing,Yi, Ganjun,Ding, Fang,Jin, Shuangxia,Hong, Ni,Wang, Guoping,Ding, Fang,Zhong, Yun,Cao, Qing,Yi, Ganjun,Cao, Qing,Hong, Ni,Wang, Guoping. 2008

[8]Cryopreservation of in vitro cultured shoot tips of strawberry. Zhao, Mizhen,Wang, Zhuangwei,Su, Jiale,Qian, Yaming. 2006

[9]Micropropagation and cryopreservation of in vitro shoot tips of 'Suizhonghong' papaya. Jiwu, Zeng,Ganjun, Yi. 2007

[10]Effect of sugars on maturation rate of vitrified-thawed immature porcine oocytes. Huang, Jing,Li, Qingwang,Li, Wenye,Xiao, Bo,Wu, Shuyun,Jiang, Zhongliang,Hu, Jianhong,Li, Qingwang,Zhao, Rui,Han, Zengsheng,Liu, Lei,Chen, Xiaoyu,Xiao, Bo. 2008

[11]POSITIVE EFFECT OF APOPTOTIC INHIBITOR Z-VAD-FMK ON VITRIFIED-THAWED PORCINE MII STAGE OOCYTES. Niu, Yingfang,Dai, Jianjun,Chen, Yaning,Wu, Caifeng,Zhang, Shushan,Zhang, Defu. 2016

[12]Cryopreservation of mango (Mangifera indica L.) embryogenic cultures. Wu, YJ,Huang, XL,Xiao, JN,Li, XJ,Zhou, MD,Engelmann, F. 2003

[13]Effect of cryotop vitrification method on oocytes at different meiotic stages and embryo development in vitro. Li Xiangchen,Yao Yaxin,Wang Yipeng,Li Heping,Guan Weijun,Ma Yuehui. 2012

[14]Effect of 5-aza-2 '-deoxycytidine on methylation of the putative imprinted control region of H19 during the in vitro development of vitrified bovine two-cell embryos. Zhao, Xue-Ming,Ren, Jing-Jing,Du, Wei-Hua,Hao, Hai-Sheng,Wang, Dong,Liu, Yan,Qin, Tong,Zhu, Hua-Bin. 2012

[15]BOTH DEATH RECEPTOR AND MITOCHONDRIA MEDIATED APOPTOTIC PATHWAYS PARTICIPATED THE OCCURRENCE OF APOPTOSIS IN PORCINE VITRIFIED MII STAGE OOCYTES. Dai, Jianjun,Niu, Yingfang,Wu, Caifeng,Zhang, Shushan,Zhang, Defu,Dai, Jianjun,Niu, Yingfang,Wu, Caifeng,Zhang, Shushan,Zhang, Defu. 2016

[16]Melatonin inhibits apoptosis and improves the developmental potential of vitrified bovine oocytes. Zhao, Xue-Ming,Hao, Hai-Sheng,Du, Wei-Hua,Zhao, Shan-Jiang,Wang, Hao-Yu,Wang, Na,Wang, Dong,Liu, Yan,Qin, Tong,Zhu, Hua-Bin. 2016

[17]Cryopreservation of apple shoot tips: Importance of cryopreservation technique and of conditioning of donor plants. Wu, YJ,Engelmann, F,Zhao, YH,Zhou, MD,Chen, SY. 1999

[18]Effect of vitrification on promoter CpG island methylation patterns and expression levels of DNA methyltransferase 1o, histone acetyltransferase 1, and deacetylase 1 in metaphase II mouse oocytes. Zhao, Xue-Ming,Ren, Jing-Jing,Du, Wei-Hua,Hao, Hai-Sheng,Wang, Dong,Qin, Tong,Liu, Yan,Zhu, Hua-Bin.

[19]PLANT-REGENERATION FROM RICE (ORYZA-SATIVA L.) EMBRYOGENIC SUSPENSION CELLS CRYOPRESERVED BY VITRIFICATION. HUANG, CN,WANG, JH,YAN, QS,ZHANG, XQ,YAN, QF. 1995

[20]Successful vitrification of mouse ovaries using less-concentrated cryoprotectants with Supercool X-1000 supplementation. Tan, Xiuwen,Song, Enliang,Liu, Xiaomu,Liu, Guifen,Cheng, Haijian,Wan, Fachun.

作者其他论文 更多>>

微信小程序