Light-regulated phosphorylation of maize phosphoenolpyruvate carboxykinase plays a vital role in its activity

文献类型： 外文期刊

第一作者： Chao, Qing

作者： Chao, Qing;Mei, Ying-Chang;Gao, Zhi-Fang;Chen, Yi-Bo;Wang, Bai-Chen;Liu, Xiao-Yu;Qian, Chun-Rong;Hao, Yu-Bo

作者机构：

关键词： Enzyme activity;Leaf sections;Light regulation;Maize;Phosphoenolpyruvate carboxykinase;Phosphorylation sites

期刊名称：PLANT MOLECULAR BIOLOGY （ 影响因子：4.076； 五年影响因子：4.89 ）

ISSN： 0167-4412

年卷期： 2014 年 85 卷 1-2 期

页码：

收录情况： SCI

摘要： Phosphoenolpyruvate carboxykinase (PEPCK)-the major decarboxylase in PEPCK-type C-4 plants-is also present in appreciable amounts in the bundle sheath cells of NADP-malic enzyme-type C-4 plants, such as maize (Zea mays), where it plays an apparent crucial role during photosynthesis (Wingler et al., in Plant Physiol 120(2):539-546, 1999; Furumoto et al., in Plant Mol Biol 41(3):301-311, 1999). Herein, we describe the use of mass spectrometry to demonstrate phosphorylation of maize PEPCK residues Ser55, Thr58, Thr59, and Thr120. Western blotting indicated that the extent of Ser55 phosphorylation dramatically increases in the leaves of maize seedlings when the seedlings are transferred from darkness to light, and decreases in the leaves of seedlings transferred from light to darkness. The effect of light on phosphorylation of this residue is opposite that of the effect of light on PEPCK activity, with the decarboxylase activity of PEPCK being less in illuminated leaves than in leaves left in the dark. This inverse relationship between PEPCK activity and the extent of phosphorylation suggests that the suppressive effect of light on PEPCK decarboxylation activity might be mediated by reversible phosphorylation of Ser55.

分类号：

  • 相关文献

[1]The Blue Light-Dependent Phosphorylation of the CCE Domain Determines the Photosensitivity of Arabidopsis CRY2. Wang, Qin,Wang, Qin,He, Reqing,Liu, Xuanming,Zhao, Xiaoying,Barshop, William D.,Vashisht, Ajay A.,Wohlschlegel, James A.,Bian, Mingdi,Liu, Bin,Wang, Qin,Yu, Xuhong,Nguyen, Paula,Lin, Chentao. 2015

[2]Phytochrome Signaling: Time to Tighten up the Loose Ends. Wang, Hai,Wang, Haiyang. 2015

[3]Identification of a UDP-glucose pyrophosphorylase from cotton (Gossypium hirsutum L.) involved in cellulose biosynthesis in Arabidopsis thaliana. Qinghua Wang,Xue Zhang,Fuguang Li,Yuxia Hou,Xingliang Liu,Xueyan Zhang.

[4]Effects of Bacterial-Feeding Nematodes and Glucose on Phenanthrene Removal by Pseudomonas putida. Jing Yongping,Li Yan,Zhang Yingpeng,Liu Ping,Sun Ming,Jing Yongping,Li Yan,Zhang Yingpeng,Jing Yongping,Liu Zhaohui,Luo, Jiafa. 2017

[5]Response of the enzymes to nitrogen applications in cotton fiber (Gossypium hirsutum L.) and their relationships with fiber strength. Wang YouHua,Feng Ying,Xu NaiYin,Chen BingLin,Ma RongHui,Zhou ZhiGuo,Xu NaiYin. 2009

[6]Physiological and morphological responses induced by alpha-particle radiation on Arabidopsis thaliana embryos. Ren, J.,Liu, L.,Fu, S. L.,Ren, J.,Jin, X. L.,Ding, Z. C.,Liu, L.. 2014

[7]Canonical correspondence analysis of soil heavy metal pollution, microflora and enzyme activities in the Pb-Zn mine tailing dam collapse area of Sidi village, SW China. Li, Zhongyi,Li, Qiang,Hu, Qingjing,Jin, Zhenjiang,Li, Zhongyi,Li, Qiang,Hu, Qingjing,Jin, Zhenjiang,Yang, Rongmei,Tang, Huafeng,Li, Min,Huang, Bingfu,Zhang, Jiayu,Li, Guiwen,Li, Zhongyi,Hu, Qingjing. 2015

[8]Sucrose and citric acid accumulations in melon genotypes with different sugar and acid contents. Tang, Mi,Zhang, Bao-cai,Xie, Jun-jun,Bie, Zhi-long,Wu, Ming-zhu,Yi, Hong-ping,Feng, Jong-xin,Tang, Mi. 2012

[9]Molecular characterization of a cathepsin F-like protease in Trichinella spiralis. Qu, Zi-gang,Ma, Xue-ting,Li, Wen-hui,Zhang, Nian-zhang,Yue, Long,Cui, Jian-min,Cai, Jian-ping,Jia, Wan-zhong,Fu, Bao-quan,Cai, Jian-ping,Jia, Wan-zhong,Fu, Bao-quan. 2015

[10]Distinct responses of soil bacterial and fungal communities to changes in fertilization regime and crop rotation. Zhang, Shuiqing,Guo, Doudou,Huang, Shaomin,Ai, Chao,Zhang, Xin,Zhou, Wei. 2018

[11]Physical changes and physiological characteristics of red and green peel during nectarine (cv. Hu018) maturation. Su, Mingshen,Chen, Kunsong,Zhang, Bo,Xu, Changjie,Sun, Chongde,Zhang, Jiukai,Li, Xian,Xi, Wanpeng,Su, Mingshen,Ye, Zhengwen,Wu, Aizhong,Guo, Juan. 2012

[12]Molecular characterization, expression and activity of glucokinase in golden pompano, Trachinotus ovatus: Response of its expression to carbohydrate in the diet. Zhou, Chuanpeng,Wang, Jun,Wang, Yun,Lin, Heizhao,Huang, Zhong,Yu, Wei. 2018

[13]The effect of long-term reclamation on enzyme activities and microbial community structure of saline soil at Shangyu, China. Liu, Chen,Xu, JianMing,Liu, Chen,Ding, NengFei,Fu, QingLin,Guo, Bin,Lin, YiCheng,Li, Hua,Li, Ningyu. 2013

[14]Soil microbial communities and enzyme activities in a reclaimed coastal soil chronosequence under rice-barley cropping. Fu, Qinglin,Liu, Chen,Ding, Nengfei,Lin, Yicheng,Guo, Bin,Luo, Jiafa,Wang, Hailong. 2012

[15]Immunolocation and enzyme activity analysis of Cryptosporidium parvum enolase. Mi, Rongsheng,Chen, Zhaoguo,Mi, Rongsheng,Yang, Xiaojiao,Huang, Yan,Cheng, Long,Lu, Ke,Han, Xiangan,Chen, Zhaoguo,Mi, Rongsheng,Yang, Xiaojiao,Huang, Yan,Cheng, Long,Lu, Ke,Han, Xiangan,Chen, Zhaoguo. 2017

[16]EFFECTS OF VITRIFICATION PROTOCOL ON THE LACTATE DEHYDROGENASE AND TOTAL ATPASE ACTIVITIES OF CHINESE MITTEN CRAB Eriocheir sinensis EMBRYOS. Huang, Xiaorong,Feng, Guangpeng,Zhao, Feng,Liu, Jianyi,Zhang, Tao,Wang, Yu,Zhuang, Ping. 2016

[17]Accumulation, histopathological effects and response of biochemical markers in the spleens and head kidneys of common carp exposed to atrazine and chlorpyrifos. Wang, Xu,Xing, Houjuan,Jiang, Yan,Xu, Shiwen,Xing, Houjuan,Sun, Gang,Wu, Hongda,Xu, Qiyou. 2013

[18]Purification and characteristics of serine protease from the head of pacific white shrimp. Wu, Yanyan,Li, Laihao,Yang, Xianqing,Diao, Shiqiang,Wang, Ping. 2012

[19]Effect of high hydrostatic pressure on overall quality parameters of watermelon juice. Liu, Y.,Zhao, X. Y.,Hu, X. S.. 2013

[20]Characterization of the putative tryptophan synthase beta-subunit from Mycobacterium tuberculosis. Shen, Hongbo,Yang, Yanping,Wang, Feifei,Xu, Shengfeng,Wang, Honghai,Zhang, Ying,Ye, Naihao. 2009

作者其他论文 更多>>

微信小程序