Design and high-level expression of a hybrid antimicrobial peptide LF15-CA8 in Escherichia coli
文献类型: 外文期刊
第一作者: Feng, Xing-Jun
作者: Feng, Xing-Jun;Xing, Li-Wei;Liu, Di;Song, Xue-Ying;Li, Jing;Xu, Wen-Shan;Liu, Chun-Long;Li, Zhong-Qiu
作者机构:
关键词: Antimicrobial peptides;LfcinB;Cecropin;Recombinant expression;Antibacterial activity
期刊名称:JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY ( 影响因子:3.346; 五年影响因子:3.426 )
ISSN: 1367-5435
年卷期: 2014 年 41 卷 3 期
页码:
收录情况: SCI
摘要: Antimicrobial peptides (AMPs) have been paid considerable attention owing to their broad-spectrum antimicrobial activity and have great potential as novel antimicrobials. In this study, a novel hybrid peptide LF15-CA8 was designed on the basis of bovine lactoferricin (LfcinB) and cecropin A. The gene segment encoding LF15-CA8 was synthesized and cloned into pGEX-4T-BH to form pGEX-4T-LC1 containing one copy of the LF15-CA8 coding region. A series of recombinant vectors containing up to six multiple-copy LF15-CA8 coding regions, i.e., pGEX-4T-LCn (n = 1-6), were subsequently constructed, and used for transformation in Escherichia coli BL21(DE3). After induction with IPTG, pGEX-4T-LC1 and pGEX-4T-LC2 transformants successfully expressed fusion proteins GST-LF15-CA8 and GST-(LF15-CA8)(2) in the form of inclusion bodies, respectively. The inclusion bodies were dissolved and the peptide was successfully released in 70 % formic acid in a single step. After purification, about 10.0 mg of the recombinant peptide LF15-CA8 with purity more than 97 % was obtained from 1 l of bacteria culture of pGEX-4T-LC2 transformants. LF15-CA8 caused an increase in antibacterial activity against Gram-positive bacterium (Staphylococcus aureus ATCC 25923) compared with the parent peptides and did not show obvious hemolytic activity against human erythrocytes in the range of effective antibacterial concentration. These results suggest that the peptide LF15-CA8 could be a promising candidate for therapeutic applications, and may lead to a cost-effective solution for the large-scale production of AMPs.
分类号:
- 相关文献
作者其他论文 更多>>
-
Metabolomic and Transcriptomic Analyses of Flavonoid Biosynthesis in Different Colors of Soybean Seed Coats
作者:Fan, Yuanfang;Wang, Xianshu;Yang, Mei;Zhong, Xiaojuan;Zhou, Yonghang;Xiang, Chao;Fan, Yuanfang;Wang, Xianshu;Yang, Mei;Zhong, Xiaojuan;Zhou, Yonghang;Xiang, Chao;Hussain, Sajad;Tao, Lei;Li, Jing
关键词:soybean; flavonoid; transcriptome; metabolome; seed coat color
-
Evaluating Sustainable Development in the Middle and Lower Reaches of the Yellow River Basin Using Multiple Data Sources
作者:Lu, Yuefeng;Li, Jing;Song, Zhenqi;Lu, Yuefeng;Lu, Miao;Lu, Yuefeng;Lu, Miao
关键词:Rivers; Indexes; Sustainable development; Socioeconomics; Remote sensing; Water resources; Urban areas; Soil; Biological system modeling; Surface treatment; Ecological environment; influencing factors; middle and lower reaches of the Yellow River Basin; social economy; sustainable development
-
PABPN1 functions as a downstream gene of CREB to inhibit the proliferation of preadipocytes
作者:Zhang, Xiao-Han;Li, Jia-Xin;Wu, Xiao-Xu;Zhang, Qian;Yang, Si-Qi;Yang, Xiu-Qin;Tian, Ming;Liu, Di
关键词:CREB; PABPN1; Preadipocyte; Proliferation; Regulatory Axis
-
Soil microbiome mediates plant community productivity in grass-legume mixtures
作者:Li, Jing;Zhang, Wenbo;Yang, Xiaojiang;Jiang, Shenyi;Wang, Zhen;Jin, Ke;Li, Jing;Xu, Zhuwen;Yang, Xiaojiang;Jiang, Shenyi;Struik, Paul C.
关键词:Grass-legume ratios; Symbiosis; Mycorrhizal fungi; Nitrogen-fixing bacteria; Plant productivity
-
Thermostability improvement of the glucose oxidase from Penicillium amagasakiense for efficient antimicrobial performance through computer-aided molecular design
作者:Peng, Ying-Zhi;Zhu, Xiao-Lu;He, Xiao-Xiao;Chen, Yi-Hao;Yang, Le-Yun;Zhao, Wei-Guo;Wang, Jun;You, Shuai;Peng, Ying-Zhi;Zhu, Xiao-Lu;He, Xiao-Xiao;Chen, Yi-Hao;Yang, Le-Yun;Zhao, Wei-Guo;Wang, Jun;You, Shuai;Lv, Xiang;Li, Jing
关键词:Penicillium amagasakiense GOD; Computer-aided molecular design; Thermostability; Flexible region; Molecular dynamics simulation; Bacteriostasis
-
Molecular mechanism of potently neutralizing human monoclonal antibodies against severe fever with thrombocytopenia virus infection
作者:Quan, Chuansong;Nie, Kaixiao;Zheng, Wenjun;Wang, Yiwen;Quan, Chuansong;Zhang, Hong;Quan, Chuansong;Ma, Dezhen;Su, Chao;Li, Lianfeng;Peng, Dingkun;Yin, Chunhong;Yang, Peipei;Shan, Chao;Liu, Xin;Zheng, Jie;Shi, Weifeng;Li, Weiwei;Gao, George F.;Qi, Jianxun;Liu, Weixiao;Liu, Di;Carr, Michael J.;Carr, Michael J.;Shi, Weifeng
关键词:severe fever with thrombocytopenia syndrome; SFTSV; Bandavirus dabieense; monoclonal antibodies; neutralization; therapeutics
-
Carbon chain elongation microorganism stimulates caproate production from ethanol and acetate under applied voltage regulation
作者:Li, Jing;Tan, Hao;Xiong, Xiaolong;Luo, Xing;Liu, He;Zhang, Xuedong;Liu, He
关键词:carbon chain elongation; optimal system; caproate; metabolism pathways; microbial community