DEVELOPMENT OF THREE-VITRIFICATION-BASED CRYOPRESERVATION PROCEDURES FOR SHOOT TIPS OF CHINA'S POTATO

文献类型： 外文期刊

第一作者： Wang, Biao

作者： Wang, Biao;Wang, Ren-rui;Li, Jing-wei;Ma, Yan-li;Wang, Qiao-chun;Wang, Ren-rui;Li, Min-fu;Sheng, Wan-min

作者机构：

关键词： Long-term conservation;droplet-vitrification;encapsulation-vitrification;Solanum tuberosum;survival;regrowth

期刊名称：CRYOLETTERS （ 影响因子：1.066； 五年影响因子：1.0 ）

ISSN： 0143-2044

年卷期： 2013 年 34 卷 4 期

页码：

收录情况： SCI

摘要： China is now the largest potato producing country worldwide. The establishment of efficient conservation techniques for potato germplasm is a prerequisite for breeding of elite cultivars. Potato viral diseases have been a great threat for sustainable potato production in China. Use of virus-free seed tubers is an effective and practical means to control viral diseases. In the present study, three vitrification-based cryopreservation techniques, i.e. droplet-vitrification, encapsulation-vitrification and vitrification were successfully developed for the first time for China's potato. Cultivar `Zihuabai' was used to optimize key parameters involved in the three vitrification-based procedures. With the optimized parameters, shoot regrowth percentages of 71%, 76% and 43% were obtained for droplet-vitrification, encapsulation-vitrification and vitrification, respectively. The three protocols developed were further tested with eight China's major cultivars, with average shoot regrowth of 61%, 38% and 28% for droplet-vitrification, encapsulation-vitrification and vitrification, respectively. Successful development of the three cryopreservation procedures using a single cultivar will facilitate a number of comparative studies such as cryo-injury, regrowth pattern, genetic stability and efficiency of virus elimination. Testing these three cryogenic procedures for potato major cultivars representing a wide range of genetic background, will help the establishment of potato cryobanking in China and the production of virus-free plants.

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