Recombinant expression, purification, and antimicrobial activity of a novel hybrid antimicrobial peptide LFT33

文献类型： 外文期刊

第一作者： Feng, Xingjun

作者： Feng, Xingjun;Guo, Jiayin;Song, Xueying;Li, Jing;Xu, Wenshan;Liu, Chunlong;Li, Zhongqiu

作者机构：

关键词： Antimicrobial peptides;Hybrid peptides;LfcinB;Thanatin;Prokaryotic expression

期刊名称：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY （ 影响因子：4.813； 五年影响因子：4.697 ）

ISSN： 0175-7598

年卷期： 2012 年 95 卷 5 期

页码：

收录情况： SCI

摘要： With great therapeutic potential against antibiotic-resistant bacteria, viruses, and even parasites, antimicrobial peptides (AMPs) have received increased interest as pharmaceutical agents in recent years. It is a worthy yet challenging work to carry out the implement and improvement of AMPs production using bioengineering techniques. In the present study, a novel hybrid peptide LFT33 was designed derived from LfcinB and thanatin. The cDNA fragment encoding LFT33 with preferred codons of Escherichia coli was chemically synthesized and ligated into the vector pET32a(+) to express the LFT33 fusion protein. The fusion protein was successfully expressed in soluble form in E. coli induced under optimized conditions. After purification by affinity chromatography, the fusion protein was cleaved successfully by enterokinase and released the peptide LFT33. About 0.5 mg of the recombinant LFT33 was obtained by reversed-phase high performance liquid chromatography from 1 l of culture medium. Mass spectrometry analysis of the purified recombinant LFT33 demonstrated that the molecular weight perfectly matched the calculated mass (4,195 Da). The recombinant peptide LFT33 caused an increase in antimicrobial activity (IC50 = 16-64 mu g/ml) against given strains and did not show hemolytic activity for human erythrocytes. The results indicated that the hybrid peptide LFT33 could serve as a promising candidate for pharmaceutical agents.

分类号：

  • 相关文献

[1]Design and high-level expression of a hybrid antimicrobial peptide LF15-CA8 in Escherichia coli. Feng, Xing-Jun,Xing, Li-Wei,Liu, Di,Song, Xue-Ying,Li, Jing,Xu, Wen-Shan,Liu, Chun-Long,Li, Zhong-Qiu. 2014

[2]Expression and characterization of recombinant gallinacin-9 and gallinacin-8 in Escherichia coli. Ma, De-ying,Shan, An-shan,Liu, Sheng-wang,Han, Zong-xi,Li, Yi-jin.

[3]Production of transgenic mice carrying the Thanatin gene by intratesticular injection. Miao, Xiang-Yang,Zhang, Xin. 2011

[4]Molecular identification and recombinant expression of red sea bream (Chrysophrys major) hepcidin gene. Chen, Song-Lin,Sui, Shao-Fei,Xu, Mei-Yu,Liu, Yang,Yang, Rong. 2005

[5]In vitro and in vivo characterization of a new recombinant antimicrobial peptide, MP1102, against methicillin-resistant Staphylococcus aureus. Zhang, Yong,Teng, Da,Wang, Xiumin,Mao, Ruoyu,Cao, Xintao,Hu, Xiaoyuan,Zong, Lifen,Wang, Jianhua,Zhang, Yong,Teng, Da,Wang, Xiumin,Mao, Ruoyu,Cao, Xintao,Hu, Xiaoyuan,Zong, Lifen,Wang, Jianhua.

[6]Bacterial expression of a Trichosanthes kirilowii defensin (TDEF1) and its antifungal activity on Fusarium oxysporum. Jian Gui-Liang,Zhang Ying-Tao,Ai Tie-Min.

[7]Mechanism of action of a novel recombinant peptide, MP1102, against Clostridium perfringens type C. Zong, Lifen,Teng, Da,Wang, Xiumin,Mao, Ruoyu,Yang, Na,Hao, Ya,Wang, Jianhua,Zong, Lifen,Teng, Da,Wang, Xiumin,Mao, Ruoyu,Yang, Na,Hao, Ya,Wang, Jianhua.

[8]Design and characterization of novel hybrid peptides from LFB15(W4,10), HP(2-20), and cecropin A based on structure parameters by computer-aided method. Tian, Zi-gang,Dong, Tian-tang,Teng, Da,Yang, Ya-lin,Wang, Jian-hua.

[9]Multimerization and fusion expression of bovine lactoferricin derivative LfcinB15-W4,10 in Escherichia coli. Tian, Zi-gang,Da Teng,Yang, Ya-lin,Luo, Jin,Feng, Xing-jun,Fan, Ying,Zhang, Fan,Wang, Jian-hua.

[10]Fusion expression of bovine lactoferricin in Escherichia coli. Feng, Xing-jun,Wang, Jian-hua,Shan, An-shan,Teng, Da,Yang, Ya-lin,Yao, Yi,Yang, Guan-pin,Shao, Yan-chun,Liu, Shuo,Zhang, Fan.

[11]EST-based in silico identification and in vitro test of antimicrobial peptides in Brassica napus. Ke, Tao,Cao, Huihui,Huang, Junyan,Dong, Caihua,Yu, Jingyin,Mao, Han,Liu, Shengyi,Ke, Tao,Wang, Xi,Niu, Qiuhong,Hui, Fengli,Huang, Junyan,Hu, Fan,Huang, Jin,Dong, Caihua,Ma, Xiangdong,Liu, Shengyi. 2015

[12]Effective Antimicrobial Activity of Plectasin-Derived Antimicrobial Peptides against Staphylococcus aureus Infection in Mammary Glands. Li, Lianbin,Gao, Yuqi,Zhao, Xin,Wang, Liangliang,Wang, Jianhua,Zhao, Xin. 2017

[13]Gene Expression Profiling of Cecropin B-Resistant Haemophilus parasuis. Wang, Chunmei,Chen, Fangzhou,Hu, Han,Li, Wentao,Chen, Pin,Liu, Yingyu,Ku, Xugang,He, Qigai,Chen, Huanchun,Wang, Chunmei,Xue, Feiqun,Wang, Yang.

[14]Two Novel Duck Antibacterial Peptides, Avian beta-Defensins 9 and 10, with Antimicrobial Activity (Retracted article. See vol. 23, pg. 1046, 2013). Liao, Wenyan,Wang, Ruiqin,Han, Zongxi,Liu, Shengwang,Ma Deying,Liao, Wenyan,Wang, Ruiqin. 2009

[15]In vitro and in vivo antibacterial effect of NZ2114 against Streptococcus suis type 2 infection in mice peritonitis models. Jiao, Jian,Mao, Ruoyu,Teng, Da,Wang, Xiumin,Hao, Ya,Yang, Na,Wang, Xiao,Wang, Jianhua,Jiao, Jian,Mao, Ruoyu,Teng, Da,Wang, Xiumin,Hao, Ya,Yang, Na,Wang, Xiao,Wang, Jianhua,Feng, Xingjun. 2017

[16]Design, Characterization and Antimicrobial Activity of Novel Antimicrobial Peptides from Temporin-Pta. Luo, Xiaofang,Qin, Yuan,He, Fulin,Qin, Zuodong,Li, Changjian,Peng, Qianrui,Gong, Zhen,Duns, Gregory J.,Liu, Wei.

[17]Bombyx mori Serpin6 regulates prophenoloxidase activity and the expression of antimicrobial proteins. Li, Bing,Yu, Hai-Zhong,Ye, Chong-Jun,Ma, Yan,Xu, Jia-Ping,Li, Bing,Ye, Chong-Jun,Li, Xing,Fan, Tao,Chen, Fu-Sheng.

[18]High-Yield Recombinant Expression of the Chicken Antimicrobial Peptide Fowlicidin-2 in Escherichia coli. Feng, Xingjun,Xu, Wenshan,Qu, Pei,Li, Xiaochong,Xing, Liwei,Liu, Di,Jiao, Jian,Wang, Jue,Li, Zhongqiu,Liu, Chunlong,Liu, Chunlong.

[19]Functional expression analysis of an acyl-ACP thioesterase FatB1 from Arachis hypogaea L. seeds in Escherichia coli. Chen, Gao,He, Qingfang,Xuan, Ning,Peng, Zhenying,Shan, Lei,Tang, Guiying,Bi, Yuping,He, Qingfang,Chen, Gao,He, Qingfang,Xuan, Ning,Peng, Zhenying,Shan, Lei,Tang, Guiying,Bi, Yuping,Chen, Gao,He, Qingfang,Xuan, Ning,Peng, Zhenying,Shan, Lei,Tang, Guiying,Bi, Yuping. 2012

[20]Identification and expression analysis of three c-type lysozymes in Oreochromis aureus. Gao, Feng-ying,Qu, Lan,Ye, Xing,Tian, Yuan-yuan,Zhang, Li-li,Bai, Jun-jie,Lu, Maixin,Qu, Lan,Ye, Xing,Yu, Shao-guo. 2012

作者其他论文 更多>>

微信小程序