Effects of long-term fertilization on nifH gene diversity in agricultural black soil

文献类型： 外文期刊

第一作者： Tang, Hui

作者： Tang, Hui;Chi, Fengqin;Wei, Dan;Tang, Hui;Tang, Hui;Yu, Miao;Wang, Yanyuan;Han, Xiaowan;Wang, Xiaogai;Jin, Wei

作者机构：

关键词： nifH;long-term fertiliser;diversity

期刊名称：AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH （ 影响因子：0.539； 五年影响因子：0.564 ）

ISSN： 1996-0808

年卷期： 2012 年 6 卷 11 期

页码：

收录情况： SCI

摘要： Black soil is a precious resource: it is an important grain-base commodity and a highly fertile soil. The long-term use of chemical and organic fertilisers has affected the microbial community and agricultural sustainability of black soil. We used nitrogenase reductase (nifH) as a biomarker of soil-borne nitrogen-fixing microbes to study the effects of long-term fertilisation on a field. Fertilisation started in 1979 and consists of the following treatments; organic, chemical, chemical and organic fertiliser combination, and no fertiliser (control treatment). The nifH gene pools from each fertilisation treatment were amplified and constructed into four clone libraries. Restriction fragment length polymorphisms (RFLP) analysis was then performed identifying 87 nifH gene types amongst the nitrogen-fixing microbial community. This analysis showed that the chemical and organic fertiliser combination resulted in a notable increase in nifH phylotypes and simultaneously intensified the differences among the unique phylotype quantities. The phylogenetic distribution of the clones based on nifH sequences from the four treatments was similar and sequences aligned with various common soils taxonomic groups, including Alphaproteobacteria, Betaproteobacteria, Deltaproteobacteria, Gammaproteobacteria, Actinobacteria, Firmicutes and Cyanobacteria. The impact of mixed fertilisers on the nifH genotypes was complex and resulted in the selection of unique microbial flora. This study also demonstrated that the addition of organic fertiliser created better environmental conditions for the composition of the nifH pool. Thus, the application of organic fertilisers can greatly increase the types of nitrogen-fixing microorganisms present and is conducive to the development of a rich variety and evolution of nitrogen fixation genes.

分类号：

  • 相关文献

[1]Genetic Diversity of Nitrogen-Fixing and Plant Growth Promoting Pseudomonas Species Isolated from Sugarcane Rhizosphere. Singh, Rajesh K.,Singh, Pratiksha,Song, Qi-Qi,Xing, Yong-Xiu,Yang, Li-Tao,Li, Yang-Rui,Li, Yang-Rui. 2017

[2]Plant Growth-Promoting Nitrogen-Fixing Enterobacteria Are in Association with Sugarcane Plants Growing in Guangxi, China. Zhang, Xincheng,Yang, Litao,Li, Yangrui,Lin, Li,Hu, Chunjin,Li, Yangrui,Lin, Li,Yang, Litao,Li, Yangrui,Li, Zhengyi,Chang, Siping,An, Qianli.

[3]Responses of soil microbial community to different concentration of fomesafen. Xu, Jun,Dong, Fengshou,Liu, Xingang,Zheng, Yongquan.

[4]Variation in the active diazotrophic community in rice paddy - nifH PCR-DGGE analysis of rhizosphere and bulk soil. Wartiainen, Ingvild,Rasmussen, Ulla,Eriksson, Torsten,Zheng, Weiwen. 2008

[5]Probing potential microbial coupling of carbon and nitrogen cycling during decomposition of maize residue by C-13-DNA-SIP. Fan, Fenliang,Yin, Chang,Li, Zhaojun,Song, Alin,Liang, Yongchao,Tang, Yongjun,Zou, Jun,Wakelin, Steven A..

[6]Isolation, characterization and phylogenetic analysis of the resistance gene analogues (RGAs) in banana (Musa spp.). Pei, Xinwu,Li, Shengjun,Jiang, Ying,Zhang, Yongqiang,Wang, Zhixing,Jia, Shirong.

[7]Influence of Different Mowing Systems on Community Characteristics and the Compensatory Growth of Important Species of the Stipa grandis Steppe in Inner Mongolia. Wan, Zhiqiang,Yang, Jiuyan,Gu, Rui,Yan, Yulong,Yang, Jie,Wan, Zhiqiang,Gu, Rui,Liang, Yan,Yan, Yulong,Gao, Qingzhu. 2016

[8]Detection of the Diversity of Cytoplasmic Male Sterility Sources in Broccoli (Brassica Oleracea var. Italica) Using Mitochondrial Markers. Shu, Jinshuai,Liu, Yumei,Li, Zhansheng,Zhang, Lili,Fang, Zhiyuan,Yang, Limei,Zhuang, Mu,Zhang, Yangyong,Lv, Honghao. 2016

[9]Field-based evidence for consistent responses of bacterial communities to copper contamination in two contrasting agricultural soils. Li, Jing,Wang, Jun-Tao,Liu, Yu-Rong,He, Ji-Zheng,Li, Jing,Wang, Jun-Tao,Ma, Yi-Bing,Hu, Hang-Wei,He, Ji-Zheng. 2015

[10]N-fertilizer-driven association between the arbuscular mycorrhizal fungal community and diazotrophic community impacts wheat yield. Zhu, Chen,Tian, Guangli,Luo, Gongwen,Kong, Yali,Guo, Junjie,Wang, Min,Guo, Shiwei,Ling, Ning,Shen, Qirong,Tian, Guangli. 2018

[11]Desmoglein 4 diversity and correlation analysis with coat color in goat. E, G. X.,Zhao, Y. J.,Na, R. S.,Zhao, Z. Q.,Jiang, C. D.,Zhang, J. H.,Chen, L. P.,Qiu, X. Y.,Hu, W.,Huang, Y. F.,Ma, Y. H.,Cao, G. L.,He, J. N.,Arlvd, S.. 2016

[12]Conservation genetics in Chinese sheep: diversity of fourteen indigenous sheep (Ovis aries) using microsatellite markers. E, Guang-Xin,Zhao, Yong-Ju,Zhang, Jia-Hua,Huang, Yong-Fu,Zhong, Tao,Ma, Yue-Hui,Gao, Hui-Jiang,He, Jian-Ning,Liu, Nan. 2016

[13]Pathotypes and Genetic Diversity of Blumeria graminis f sp hordei in the Winter Barley Regions in China. Zhu Jing-huan,Wang Jun-mei,Jia Qiao-jun,Yang Jian-ming,Lin Feng,Hua Wei,Shang Yi,Zhu Jing-huan,Zhou Yi-jun,Zhou Yi-jun. 2010

[14]Illumina-based analysis of bacterial diversity related to halophytes Salicornia europaea and Sueada aralocaspica. Sh, Ying-wu,Lou, Kai,Li, Chun,Wang, Lei,Zhao, Zhen-yong,Zhao, Shuai,Tian, Chang-yan. 2015

[15]Exploiting the diversity of Viviparous-1 gene associated with pre-harvest sprouting tolerance in European wheat varieties. Xia, L. Q.,Roeder, M. S.,Xia, L. Q.,He, Z. H.,Yang, Y.,Ganal, M. W.,Shewry, P. R.. 2008

[16]Phenotypic traits and diversity of different 100-seed weight accessions of wild soybean (Glycine soja Sieb. & Zucc.) in China. Yan, X.,Li, J.,Guo, W.,Liu, X.,Zhang, L.,Dong, Y.. 2017

[17]LTR-retrotransposon diversity and transcriptional activation under phytoplasma stress in Ziziphus jujuba. Sun, Jun,Huang, Yao,Guo, Jing,Zhou, Junyong,Sun, Qibao. 2013

[18]Random amplified polymorphism of DNA analysis in Aegilops tauschii. Kong, LR,Dong, YC,Jia, JZ. 1998

[19]Diversity maintenance and use of Vicia faba L. genetic resources. Duc, Gerard,Bao, Shiying,Baum, Michael,Redden, Bob,Sadiki, Mohammed,Jose Suso, Maria,Vishniakova, Margarita,Zong, Xuxiao. 2010

[20]Diversity and Genetic Differentiation of the Whitefly Bemisia tabaci Species Complex in China Based on mtCOI and cDNA-AFLP Analysis. Guo Xiao-jun,Rao Qiong,Zhang Fan,Luo Chen,Guo Xiao-jun,Gao Xi-wu,Rao Qiong,Zhang Hong-yu. 2012

作者其他论文 更多>>

微信小程序