New mutations existing in the nucleocapsid (N) gene of non-porcine TGEV strains isolated in China

文献类型： 外文期刊

第一作者： Yu, Xiaolong

作者： Yu, Xiaolong;Man, Chaolai

作者机构：

关键词： N gene;cloning;TGEV;non-porcine;phylogenetic analysis;homology modeling

期刊名称：AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH （ 影响因子：0.539； 五年影响因子：0.564 ）

ISSN： 1996-0808

年卷期： 2011 年 5 卷 30 期

页码：

收录情况： SCI

摘要： Transmissible gastroenteritis virus (TGEV) is the etiological agent of TGE, and non-porcine hosts are potential carriers of TGEVs. In this study, genomic RNA were extracted from TGEVs designated HYM-09, HYM-09-1 and HYM-09-2 isolated respectively from non-porcine hosts (dogs, cats and foxes) naturally infected with TGEVs. The nucleocapsid (N) genes of the three non-porcine TGEV strains were amplified by RT-PCR and cloned into pMD18-T vector respectively. Each N cDNA was sequenced and encompassed an open reading frame of 1149 nucleotides, encoding a 382-amino acids protein. Sequence analyses of the N genes were performed, including homologous comparison, phylogenetic tree analysis and homology modeling. The results showed that there existed some new point mutations in the three non-porcine TGEV isolates N genes. The phylogenetic tree analysis revealed that these non-porcine TGEVs N genes had closer genetic relationships and evolution distance to the N genes of Chinese TGEV strains. The three-dimensional structure of the N protein (32-154AA (amino acid)) of HYM-09-2 generated by homology modeling was different from that of other TGEV strains (including HYM-09 and HYM-09-1). These data serve as a foundation for further insight into the virulence or mutation rates of the non-porcine TGEVs.

分类号：

  • 相关文献

[1]Cloning and Sequence Analysis of N Gene of Transmissible Gastroenteritis Virus HYM-09 Isolated from Dog in China. Yu, Xiaolong,Man, Chaolai. 2012

[2]A sensitive duplex nanoparticle-assisted PCR assay for identifying porcine epidemic diarrhea virus and porcine transmissible gastroenteritis virus from clinical specimens. Zhu, Yu,Wang, Chunren,Cui, Yudong,Cui, Shangjin,Zhu, Yu,Liang, Lin,Luo, Yakun,Cui, Shangjin,Wang, Guihua,Ai, Xia.

[3]Characterization of a new cry2Ab gene of Bacillus thuringiensis with high insecticidal activity against Plutella xylostella L.. Pan, Zhizhen,Zhu, Yujing,Shi, Huai,Chen, Zheng,Chen, Meichun,Liu, Bo,Xu, Lian,Chen, Qingxi.

[4]Expression, purification and molecular characterization of a novel endoglucanase protein from Bacillus subtilis SB13. Guan, Xuefang,Chen, Penglian,Xu, Qingxian,Qian, Lei,Huang, Juqing,Lin, Bin.

[5]Identification, distribution pattern of IS231 elements in Bacillus thuringiensis and their phylogenetic analysis. Liu, JJ,Song, FP,Shu, CL,Qiu, JZ,Guan, X,Huang, DF,Zhang, J.

[6]Molecular cloning and expression analysis of annexin A2 gene in sika deer antler tip. Xia, Yanling,Qu, Haomiao,Lu, Binshan,Zhang, Qiang,Li, Heping,Xia, Yanling. 2018

[7]Molecular characterization of a new begomovirus infecting Senecio scandens in China. Zhou, XP,Cai, JH,Li, GX. 2005

[8]Effective inhibition of porcine transmissible gastroenteritis virus replication in ST cells by shRNAs targeting RNA-dependent RNA polymerase gene. Zhou, Jun-fang,Hua, Xiu-guo,Cui, Li,Zhu, Jian-guo,Miao, De-nian,Zou, Yong,He, Xi-zhong,Su, Wan-guo. 2007

[9]Inhibition of porcine transmissible gastroenteritis virus (TGEV) replication in mini-pigs by shRNA. Zhou, Junfang,Huang, Fen,Hua, Xiuguo,Cui, Li,Zhang, Wen,Shen, Yan,Yan, Yijia,Chen, Piren,Mou, Jing,Lan, Daoliang,Yang, Zhibiao,Zhou, Junfang,Ding, Dezhong,Chen, Qi. 2010

[10]Identification of cellular proteome using two-dimensional difference gel electrophoresis in ST cells infected with transmissible gastroenteritis coronavirus. Zhang, Xin,Shi, Hong-Yan,Chen, Jian-Fei,Shi, Da,Feng, Li,Lang, Hong-Wu,Wang, Zhong-Tian. 2013

[11]A SCAR marker linked to the N gene for resistance to root knot nematodes (Meloidogyne spp.) in pepper (Capsicum annuum L.). Wang, L. H.,Gu, X. H.,Hua, M. Y.,Mao, S. L.,Zhang, Z. H.,Zhang, B. X.,Gu, X. H.,Hua, M. Y.,Yun, X. F.,Peng, D. L..

[12]Development of an Epitope-Based Competitive ELISA for the Detection of Antibodies against Tibetan Peste des Petits Ruminants Virus. Yu, Rui-Song,Zhu, Yu-Min,Dong, Shi-Juan,Li, Zhen,Zhang, Guo-Rui,Yu, Rui-Song,Zhu, Yu-Min,Dong, Shi-Juan,Li, Zhen,Zhang, Guo-Rui,Lei, Zhi-Hai,Zeng, Jiang-Yong,Zhu, Se,Duoji, Ciren,Dunzhu, Luobu. 2013

[13]Development and application of a molecular marker for TMV resistance based on N gene in tobacco (Nicotiana tabacum). Zhang Yu,Wen Liuying,Yang Aiguo,Luo Chenggang,Cheng Lirui,Jiang Caihong,Chang Aixia,Wang Yuanying,Li Wei,Zhang Jing,Xiao Zhixin.

[14]Confirmation and Sequence analysis of N gene of PPRV in South Xinjiang, China. Liu, YongHong,Li, XianQiang,Zhang, Luyao,Li, Fei,Zhao, Li,Yang, Bo,Wang, XueFeng. 2017

[15]Rapid differentiation of vaccine strain and Chinese field strains of transmissible gastroenteritis virus by restriction fragment length polymorphism of the N gene. Wang, Chengbao,Chen, Jianfei,Shi, Hongyan,Qiu, Hua-Ji,Xue, Fei,Liu, Shengwang,Liu, Changming,Zhu, Yuanmao,Feng, Li,Almazan, Fernando,Enjuanes, Luis. 2010

[16]Functional analyses of the chitin-binding domains and the catalytic domain of Brassica juncea chitinase BjCHI1. Tang, CM,Chye, ML,Ramalingam, S,Ouyang, SW,Zhao, KJ,Ubhayasekera, W,Mowbray, SL. 2004

[17]Molecular characterization and expression analysis of beta(2)-microglobulin in large yellow croaker Pseudosciaena crocea. Yu, Suhong,Chen, Xinhua,Ao, Jingqun,Yu, Suhong,Yu, Suhong. 2009

[18]Allelic variation in TLR4 is linked to resistance to Salmonella Enteritidis infection in chickens. Zhao, Xingwang,Liu, Ranran,Song, Yongmei,Li, Qinghe,Zheng, Maiqing,Cui, Huanxian,Zhao, Guiping,Wen, Jie,Li, Peng,Everaert, Nadia,Wang, Huihua,Gou, Zhongyong.

[19]Exploring the Molecular Basis for Binding of Inhibitors by Threonyl-tRNA Synthetase from Brucella abortus: A Virtual Screening Study. Li, Ming,Wen, Fang,Zhao, Shengguo,Wang, Pengpeng,Li, Songli,Zhang, Yangdong,Zheng, Nan,Wang, Jiaqi,Li, Ming,Wen, Fang,Zhao, Shengguo,Wang, Pengpeng,Li, Songli,Zhang, Yangdong,Zheng, Nan,Wang, Jiaqi,Zheng, Nan,Wang, Jiaqi. 2016

[20]Phylogenetic and structural analysis of major surface proteins hemagglutinin and neuraminidase of novel avian influenza virus A H7N9 from chinese patient. Yuan Xiao-hui,Wang Ying-chen,Qu Zhang-yi,Yuan Xiao-hui,Ren Jia-yi,Yuan Xiao-hui,Ren Jia-yi,Wu Xiao-min,Wang Jing-fei. 2013

作者其他论文 更多>>

微信小程序