Cloning Na+/H+ Antiporter Gene (nhaA) and Analysis of Function in Soybean

文献类型： 外文期刊

第一作者： Wang Quanwei

作者： Wang Quanwei;Chen Liang;Zhang Hailing

作者机构：

关键词： Na+/H+ antiporter gene(nhaA);cloning;soybean;genetic transformation;salt tolerance

期刊名称：ENVIRONMENTAL BIOTECHNOLOGY AND MATERIALS ENGINEERING, PTS 1-3

ISSN： 1022-6680

年卷期： 2011 年 183-185 卷

页码：

收录情况： SCI

摘要： The osmotic stress and ion toxicity caused by high salt is one of the major abiotic stress factors that affect the crop growth and development. Na+/H+ antiporter is the key factor in the salt-stress tolerance in organism. It can maintain normal growth and metabolism of organism under high salt stress. PCR was per- formed to clone a plasma membrane Na+/H+ antiporter gene nhaA from Pseudomonas stutzeri, and the plant expression vector pBI121-nhaA was constructed. NhaA gene was trans- formed into soybean growing point via Agrobacterium- mediated transformation. PCR, Southern blot analysis showed that nhaA gene had been integrated into the soybean genome. Salt resistance analysis showed that the relative electronic conductivity of the transformed plants plasma membrane was signify- cantly lower than that of the control under salt stress. While the content of chlorophyll and proline in the transformed plants were significantly higher than that in the controls. The expression of nhaA gene increased the salt stress tolerance of the transgenic soybean and provided excellent candidate genes for improving salt tolerance of crops.

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