Effect of Cycloheximide on the Development of Parthenogenetic Activation and Cloned Porcine Embryos

文献类型： 外文期刊

第一作者： Ma, Hong

作者： Ma, Hong;Fu, Bo;Li, Zhongqiu;Liu, Di;Zhao, Jinfeng;Fang, Qingchang;Ren, Liang

作者机构：

关键词： cycloheximide;parthenogenetic activation;somatic cell nuclear transfer

期刊名称：ISBE 2011: 2011 INTERNATIONAL CONFERENCE ON BIOMEDICINE AND ENGINEERING, VOL 2

ISSN：

年卷期： 2011 年

页码：

收录情况： SCI

摘要： The purpose of this study was to investigate the effect of cycloheximide on the development of parthenogenetic activation and somatic cell nuclear transfered porcine embryos. porcine oocytes (44h after IVM) treated using electrical activation combinations of cycloheximide or cytochalsin B or 6-dimethylaminipurine were evaluated to determine the development rates of parthenogenetic activation porcine embryos (Group 1).Pretreatment with cycloheximide before electrical activation combinations of different reagents were evaluated to determine the development rates of parthenogenetic activation porcine embryos (Group 2).The groups were comprised of the treated using electrical activation combinations of different reagents were evaluated to determine the development rates of cloned porcine embryos (Group 3).The results show that the cycloheximide exhibited significantly (P<0.05) lower rates of parthenogenetic activation cleavage rates and of blastocyst rates, and longer time of parthenogenetic activation. However, Pre-treatment with cycloheximide significantly increased parthenogenetic activation cleavage rates and of blastocyst rates. Treated using electrical activation combinations of cycloheximide had the highest blastocyst yield of cloned porcine embryos.

分类号：

  • 相关文献

[1]Two imide substances from a soil-isolated Streptomyces atratus strain provide effective biocontrol activity against grapevine downy mildew. Liang, Chunhao,Liang, Chunhao,Zang, Chaoqun,Zhao, Kuihua,Yu, Shuyi,McDermott, Mark I.,Huang, Yuqian.

[2]Changes in the 3-dimensional distribution of mitochondria during meiotic divisions of mouse oocytes. Li, CJ,Fan, BQ. 1997

[3]Expression of Interferon-tau mRNA in Bovine Embryos Derived from Different Procedures. Yao, N.,Wan, P-C,Hao, Z-D,Gao, F-F,Yang, L.,Cui, M-S,Wu, Y.,Liu, S.,Chen, H.,Zeng, S-M,Yao, N.,Wan, P-C.

[4]In vitro maturation and artificial activation of donkey oocytes. Wu, Kaifeng,Cui, Liang,Zhao, Lixia,Liu, Yiyi,Zhou, Huanmin,Zhao, Gaoping,Tan, Xiuwen.

[5]Treating Cloned Embryos, But Not Donor Cells, with 5-aza-2 '-deoxycytidine Enhances the Developmental Competence of Porcine Cloned Embryos. Huan, Yan Jun,Zhu, Jiang,Xie, Bing Teng,Wang, Jian Yu,Liu, Shi Chao,Zhou, Yang,Kong, Qing Ran,Liu, Zhong Hua,Huan, Yan Jun,He, Hong Bin. 2013

[6]Alteration of the DNA methylation status of donor cells impairs the developmental competence of porcine cloned embryos. Huan, Yan Jun,Zhu, Jiang,Xie, Bing Teng,Wang, Jian Yu,Li, Jing Yu,Xue, Bing Hua,Kong, Qing Ran,Liu, Zhong Hua,Huan, Yan Jun,Wu, Zhan Feng,Zhang, Ji Guang.

[7]Positive effects of treatment of donor cells with aphidicolin on the preimplantation development of somatic cell nuclear transfer embryos in Chinese Bama mini-pig (Sus Scrofa). Zhang, Ting-Yu,Dai, Jian-Jun,Wu, Cai-Feng,Gu, Xiao-Long,Liu, Liang,Wu, Zhi-Qiang,Xie, Yi-Ni,Wu, Bin,Chen, Hui-Lan,Zhang, De-Fu,Zhang, Ting-Yu,Dai, Jian-Jun,Wu, Cai-Feng,Gu, Xiao-Long,Liu, Liang,Wu, Zhi-Qiang,Xie, Yi-Ni,Wu, Bin,Chen, Hui-Lan,Zhang, De-Fu,Li, Yao,Chen, Xue-Jin,Gu, Xiao-Long,Wu, Zhi-Qiang,Xie, Yi-Ni,Wu, Bin. 2012

[8]Generation of chimeric minipigs by aggregating 4-to 8-cell-stage blastomeres from somatic cell nuclear transfer with the tracing of enhanced green fluorescent protein. Ji, Huili,Long, Chuan,Feng, Chong,Shi, Ningning,Jiang, Yingdi,Zeng, Guomin,Li, Xirui,Wu, Jingjing,Pan, Dengke,Ji, Huili,Lu, Shengsheng,Long, Chuan,Lu, Lin. 2017

[9]Optimizing of Activation Protocols and Production of Transgenic Pigs Expressing Human Lysozyme by Somatic Cell Nuclear Transfer. Wei Heng-Xi,Gao Feng-Lei,Zhang Shou-Quan,Wei Heng-Xi,Li Jun,Tong Jia,Ma Yu-Fang,Li Qiu-Yan,Li Ning,Wei Heng-Xi,Li Jun,Tong Jia,Ma Yu-Fang,Li Qiu-Yan,Li Ning,Li Jun. 2013

[10]Screening somatic cell nuclear transfer parameters for generation of transgenic cloned cattle with intragenomic integration of additional gene copies that encode bovine adipocyte-type fatty acid-binding protein (A-FABP). Guo, Yong,Sheng, Xihui,Chang, Di,Qi, Xiaolong,Wang, Xiangguo,Liu, Yunhai,Ni, Hemin,Li, Hejuan,Wang, Ying,Yan, Xingrong,Li, Junya.

[11]Epigenetic Modification Agents Improve Genomic Methylation Reprogramming in Porcine Cloned Embryos. Huan, Yan Jun,Wang, Hong Mei,Bin He, Hong,Huan, Yan Jun,Zhu, Jiang,Xie, Bing Teng,Li, Jing Yu,Kong, Qing Ran,Liu, Zhong Hua,Zhu, Jiang,Wu, Zhan Feng,Zhang, Ji Guang.

[12]The high-level accumulation of n-3 polyunsaturated fatty acids in transgenic pigs harboring the n-3 fatty acid desaturase gene from Caenorhabditis briggsae. Zhou, Yanrong,Lin, Yanli,Wu, Xiaojie,Xiong, Fuyin,Chen, Hongxing,Feng, Chong,Long, Chuan,Wang, Ning,Pan, Dengke. 2014

[13]Genomic Imprinting of GTL2 in Somatic Cell Nuclear Transfer Cattle. Wang Jian-Chun,Fan Bao-Liang,Li Shi-Jie,Li Ning,Li Shi-Jie,Li Dong-Jie,Du Wei-Hua. 2008

[14]Multi-transgenic minipig models exhibiting potential for hepatic insulin resistance and pancreatic apoptosis. Kong, Siyuan,Ruan, Jinxue,Xin, Leilei,Fan, Junhua,Xia, Jihan,Liu, Zhiguo,Mu, Yulian,Yang, Shulin,Li, Kui.

[15]Effect of Pyruvate and Lactic Acid on the Development of Porcine Embryos. Fu Bo,Llu Di,Ma Hong,Li Zhong-qiu,Wang Liang,Bai Jing. 2012

[16]Development of porcine tetraploid somatic cell nuclear transfer embryos is influenced by oocyte nuclei. Fu, Bo,Liu, Di,Ma, Hong,Guo, Zhen-Hua,Wang, Liang,Li, Zhong-Qiu,Peng, Fu-Gang,Liu, Di,Bai, Jing.

[17]Timing of The First Zygotic Cleavage as a Developmental Potential Marker for Porcine Cloned Embryos. Luo Xue-Ming,Feng Chong,Long Chuan,Yan Jun,Pan Deng-Ke,Xiao Wei,Xue Zhen-Hua,Yun Peng. 2010

[18]Generation of porcine fetal fibroblasts expressing the tetracycline-inducible Cas9 gene by somatic cell nuclear transfer. Liu, Guoqian,Liu, Kai,Wei, Hengxi,Li, Li,Zhang, Shouquan,Liu, Guoqian,Liu, Kai,Wei, Hengxi,Li, Li,Zhang, Shouquan.

[19]Effect of trichostatin A on transfected donor cells and subsequent development of porcine cloned embryos. Liu Di,Ma Hong,Wang Liang,Guo Zhen-hua,Li Zhong-qiu,Fu Bo,Liu Di,Fang Qing-chang,Ren Liang.

[20]Production of transgenic dairy goat expressing human alpha-lactalbumin by somatic cell nuclear transfer. Feng, Xiujing,Cao, Shaoxian,Wang, Huili,Meng, Chunhua,Li, Jingxin,Jiang, Jin,Qian, Yong,Su, Lei,He, Qiang,Zhang, Qingxiao,Feng, Xiujing,Li, Jingxin,Jiang, Jin,Su, Lei,Zhang, Qingxiao,Feng, Xiujing,Feng, Xiujing,Cao, Shaoxian,Wang, Huili,Meng, Chunhua,Qian, Yong.

作者其他论文 更多>>

微信小程序