Development of SYBR Green I-based quantitative PCR assay for identification of porcine circovirus 1, 2 and 3

文献类型： 外文期刊

第一作者： Liu, Chang

作者： Liu, Chang;Tian, Yao;Shi, Jian-Li;Li, Chen;Xu, Shao-Jian;Wu, Xiao-Yan;Han, Hong;Li, Jun;Tian, Yao;Zheng, Li-mei;Ma, Ying-Ru;Li, Jun;Han, Xian-Jie

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关键词： porcine circovirus; specificity; sensitivity; SYBR Green I based quantitative PCR; single identification method

期刊名称：VETERINARSKI ARHIV （ 影响因子：0.5； 五年影响因子：0.6 ）

ISSN： 0372-5480

年卷期： 2023 年 93 卷 4 期

页码：

收录情况： SCI

摘要： Porcine Circovirus (PCV) includes Porcine Circovirus 1(PCV1), Porcine Circovirus 2 (PCV2) and Porcine Circovirus 3 (PCV3). In recent years, co-infection exists between PCV1, PCV2 and PCV3 serotypes. Therefore, it is particularly necessary to establish a fast, specific and sensitive SYBR Green I real-time quantitative PCR detection method for PCV1, PCV2 and PCV3. In this experiment, specific primers were selected and the reaction conditions were optimized. A real-time quantitative PCR identification method was established. The results showed the detection limits of this assay were 40.3 copies/mu l for PCV1, 25.2 copies/mu l for PCV2 and22.4 copies/mu l for PCV3. There was no cross-reactivity with swine fever virus (CSFV), porcine reproductive and respiratory syndrome virus (PRRSV), porcine pseudorabies virus (PRV) and porcine parvovirus (PPV). The intra-assay and inter-assay coefficients of variation were less than 1%. The test results of 100 PCV suspected positive samples revealed that the PCV1, PCV2 and PCV3 singular infection rate was 10% (10/100), 64% (64/100) and 52% (52/100), respectively. The PCV1 and PCV2 co-infection rate was 8% (8/100), the PCV1 and PCV3 co-infection rate was 7% (7/100), the PCV2 and PCV3 co-infection rate was 26% (26/100), and the PCV1, PCV2 and PCV3 co-infection rate was 7% (7/100). This method has good specificity, sensitivity and stability. It provides a promising tool for rapid differential detection of PCV1, PCV2 and PCV3.

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