Construction of an infectious full-length and eGFP-tagged cDNA clone of a chilli ringspot virus isolate from Yunnan province, China
文献类型： 外文期刊
第一作者： Hua, Mengying
作者： Hua, Mengying;Lu, Yuwen;Zheng, Hongying;Chen, Jianping;Yan, Fei;Peng, Jiejun;Jiang, Shanshan;Wang, Liyan;Yuan, Enping;Wang, Shaoxiang;Wan, Qionglian;Chen, Hairu
作者机构：
期刊名称：ARCHIVES OF VIROLOGY （ 影响因子：2.685； 五年影响因子：2.506 ）
ISSN： 0304-8608
年卷期： 2022 年 167 卷 7 期
页码：
收录情况： SCI
摘要： Chilli ringspot virus (ChiRSV; genus Potyvirus) was one of several viruses previously detected in pepper samples with severe yellowing and curling symptoms growing in Wenshan, Yunan province, China. We now report the full-length sequence of ChiRSV-YN/Wenshan (MZ269480), which has 88.5-98.9% nucleotide sequence identity to other published ChiRSV isolates. A full-length cDNA infectious clone was constructed. This cDNA and an eGFP-tagged clone were infectious, leading to systemic symptoms in both Nicotiana benthamiana and Capsicum spp. Recombinant clones containing the P1 protein coding region of other ChiRSV isolates differed in their pathogenicity. Single infection by ChiRSV caused mild mosaic or leaf crinkling in Capsicum frutescens L. and Capsicum annuum L.
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