Large DNA fragment deletion in lncRNA77580 regulates neighboring gene expression in soybean (Glycine max)

文献类型： 外文期刊

第一作者： Niu, Fengjuan

作者： Niu, Fengjuan;Jiang, Qiyan;Sun, Xianjun;Hu, Zheng;Wang, Lixia;Zhang, Hui

作者机构：

关键词： CRISPR; Cas9; gene editing; gene expression; hairy root; large DNA fragment deletion; long non-coding RNA; salt stress; soybean

期刊名称：FUNCTIONAL PLANT BIOLOGY （ 影响因子：3.101； 五年影响因子：3.248 ）

ISSN： 1445-4408

年卷期： 2021 年 48 卷 11 期

页码：

收录情况： SCI

摘要： Long non-coding RNAs (lncRNAs) affect gene expressions via a wide range of mechanisms and are considered important regulators of numerous essential biological processes, including abiotic stress responses. However, the biological functions of most lncRNAs are yet to be determined. Moreover, to date, no effective methods have been developed to study the function of plant lncRNAs. We previously discovered a salt stress-related lncRNA, lncRNA77580 in soybean (Glycine max L.). In this study, we cloned the full-length lncRNA77580 and found that it shows nuclear-specific localisation. Furthermore, we employed CRISPR/Cas9 technology to induce large DNA fragment deletions in lncRNA77580 in soybean using a dual-single guide RNA/Cas9 design. As a result, we obtained deletion mutant soybean roots with targeted genomic fragment deletion in lncRNA77580. Deletion and overexpression of lncRNA77580 were found to alter the expression of several neighboring protein-coding genes associated with the response to salt stress. The longer the deleted DNA fragment in lncRNA77580, the greater the influence on the expression of lncRNA77580 itself and neighboring genes. Collectively, the findings of this study revealed that large DNA fragment deletion in lncRNAs using the CRISPR/Cas9 system is a powerful method to obtain functional mutations of soybean lncRNAs that could benefit future research on lncRNA function in soybean.

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