Cytokinin-inducible response regulator SlRR6 controls plant height through gibberellin and auxin pathways in tomato

文献类型： 外文期刊

第一作者： Liu, Yue

作者： Liu, Yue;Liu, Yichen;Yan, Yanqiu;Yu, Xiaolin;Ali, Muhammad;Pan, Changtian;Lu, Gang;He, Yanjun;Lu, Gang

作者机构：

关键词： Auxin; cytokinin signaling; gibberellin; plant height; response regulator; SAUR; tomato

期刊名称：JOURNAL OF EXPERIMENTAL BOTANY （ 影响因子：6.9； 五年影响因子：8.0 ）

ISSN： 0022-0957

年卷期： 2023 年 74 卷 15 期

页码：

收录情况： SCI

摘要： SlRR6, a type-A response regulator in the cytokinin signal transduction system, interacts with the auxin-responsive gene SlSAUR58to regulate tomato plant height through integrating gibberellin and auxin homeostasis. Plant height is a key agronomic trait regulated by several phytohormones such as gibberellins (GAs) and auxin. However, little is known about how cytokinin (CK) participates in this process. Here, we report that SlRR6, a type-A response regulator in the CK signaling pathway, positively regulates plant height in tomato. SlRR6 was induced by exogenous kinetin and GA(3), but inhibited by indole-3-acetic acid (IAA). Knock out of SlRR6 reduced tomato plant height through shortening internode length, while overexpression of SlRR6 caused taller plants due to increased internode number. Cytological observation of longitudinal stems showed that both knock out and overexpression of SlRR6 generated larger cells, but significantly reduced cell numbers in each internode. Further studies demonstrated that overexpression of SlRR6 enhanced GA accumulation and lowered IAA content, along with expression changes in GA- and IAA-related genes. Exogenous paclobutrazol and IAA treatments restored the increased plant height phenotype in SlRR6-overexpressing lines. Yeast two-hybrid, bimolecular fluorescence complementation, and co-immunoprecipitation assays showed that SlRR6 interacts with a small auxin up RNA protein, SlSAUR58. Moreover, SlSAUR58-overexpressing plants were dwarf with decreased internode length. Overall, our findings establish SlRR6 as a vital component in the CK signaling, GA, and IAA regulatory network that controls plant height.

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