Immunomodulatory Effects of Microcin C7 in Cyclophosphamide-Induced Immunosuppressed Mice

文献类型： 外文期刊

第一作者： Dai, Zi-qi

作者： Dai, Zi-qi;Shang, Li-jun;Zeng, Xiang-fang;Wang, Xin-yu;Li, Si-yu;Qiao, Shiyan;Yu, Haitao;Dai, Zi-qi;Shang, Li-jun;Zeng, Xiang-fang;Wang, Xin-yu;Li, Si-yu;Qiao, Shiyan;Yu, Haitao;Wei, Yu-shu;Li, Ze-qiang;Chen, Mei-xia

作者机构：

关键词： Microcin C7; cyclophosphamide; immunomodulatory; intestinal mucosal immunity; intestinal epithelial barrier; gut microflora

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：6.1； 五年影响因子：6.3 ）

ISSN： 0021-8561

年卷期： 2023 年

页码：

收录情况： SCI

摘要： Microcin C7 (McC) as a viable immunomodulator peptidecan be apotential solution for pathogenic microbial infection in the post-antibioticera and has gained substantial attention. This study was designedto evaluate the immunomodulatory activity of Microcin C7 in a cyclophosphamide(CTX)-induced immunodeficient mouse model. We show that Microcin C7treatment significantly alleviated the CTX-caused body weight loss,improved the feed and water consumption to improve the state of themice, and elevated the absolute number and proportion of peripheralblood lymphocytes as well as the level of hemoglobulin. We furtheraim to characterize the phenotypes of the immune function and intestinalhealth profiles. The results demonstrate that Microcin C7 treatmentincreased serum levels of immunoglobulin A (IgA), IgG, interleukin6, and hemolysin, promoted splenic lymphocyte proliferation inducedby concanavalin A and LPS, and enhanced the phagocytosis of peritonealmacrophages immunized by sheep red blood cells. Additionally, MicrocinC7 treatment decreased levels of diamine oxidase and d-lactate,ameliorated CTX-induced intestinal morphological damage, and increasedthe levels of zonula occluden 1, occludin, claudin-1, mucin 2, andsecretary IgA in the jejunum and colon. Moreover, Microcin C7 administrationis sufficient to reverse CTX-induced intestinal microbiota dysbiosisby increasing the number of Lactobacillus and Bifidobacterium, decreasing thenumber of Escherichia coli in coloniccontents. Collectively, our results demonstrate that Microcin C7 mayhave protective and immunomodulatory functions and could be a potentialcandidate used in animal feed, functional foods, and immunologicalregimens..

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