A Genome-Wide Analysis of Serine Protease Inhibitors in Cydia pomonella Provides Insights into Their Evolution and Expression Pattern

文献类型： 外文期刊

第一作者： Wu, Qiang

作者： Wu, Qiang;Huang, Cong;Liu, Bo;Wan, Fanghao;Qian, Wanqiang;Xing, Longsheng;Du, Min;Zhou, Hongxu;Liu, Wanxue

作者机构： Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Minist Agr & Rural Affairs, Shenzhen Branch,Guangdong Lab Lingnan Modern Agr,G, Shenzhen 518120, Peoples R China;Hebei Univ, Inst Life Sci & Green Dev, Coll Life Sci, Hebei Basic Sci Ctr Biot Interact, Baoding 071000, Peoples R China;Qingdao Agr Univ, Sino Australian Joint Res Inst Agr & Environm Hlth, Coll Plant Hlth & Med, Shandong Prov Key Lab Integrated Control Plant Dis, Qingdao 266109, Peoples R China;Chinese Acad Agr Sci, State Key Lab Biol Plant Dis & Insect Pests, Inst Plant Protect, Beijing 100193, Peoples R China

关键词： serine protease inhibitor; melanization; Cydia pomonella; innate immunity; alternative splicing

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 2022影响因子：5.6； 五年影响因子：6.2 ）

ISSN： 1661-6596

年卷期： 2023 年 24 卷 22 期

页码：

收录情况： SCI

摘要： Serine protease inhibitors (serpins) appear to be ubiquitous in almost all living organisms, with a conserved structure and varying functions. Serpins can modulate immune responses by negatively regulating serine protease activities strictly and precisely. The codling moth, Cydia pomonella (L.), a major invasive pest in China, can cause serious economic losses. However, knowledge of serpin genes in this insect remain largely unknown. In this study, we performed a systematic analysis of the serpin genes in C. pomonella, obtaining 26 serpins from the C. pomonella genome. Subsequently, their sequence features, evolutionary relationship, and expression pattern were characterized. Comparative analysis revealed the evolution of a number of serpin genes in Lepidoptera. Importantly, the evolutionary relationship and putative roles of serpin genes in C. pomonella were revealed. Additionally, selective pressure analysis found amino acid sites with strong evidence of positive selection. Interestingly, the serpin1 gene possessed at least six splicing isoforms with distinct reactive-center loops, and these isoforms were experimentally validated. Furthermore, we observed a subclade expansion of serpins, and these genes showed high expression in multiple tissues, suggesting their important roles in C. pomonella. Overall, this study will enrich our knowledge of the immunity of C. pomonella and help to elucidate the role of serpins in the immune response.

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