Fullerenol Quantum Dots-Based Highly Sensitive Fluorescence Aptasensor for Patulin in Apple Juice

文献类型： 外文期刊

第一作者： Pang, Hua

作者： Pang, Hua;Li, Hui;Zhang, Wen;Mao, Jin;Zhang, Liangxiao;Zhang, Zhaowei;Zhang, Qi;Wang, Du;Jiang, Jun;Li, Peiwu;Pang, Hua;Li, Hui;Zhang, Wen;Zhang, Qi;Li, Peiwu;Pang, Hua;Li, Hui;Zhang, Wen;Mao, Jin;Zhang, Liangxiao;Zhang, Zhaowei;Zhang, Qi;Li, Peiwu;Li, Hui;Zhang, Wen;Mao, Jin;Zhang, Zhaowei;Zhang, Qi;Li, Peiwu;Li, Hui;Zhang, Wen;Mao, Jin;Zhang, Liangxiao;Zhang, Zhaowei;Zhang, Qi;Wang, Du;Jiang, Jun;Li, Peiwu

作者机构：

关键词： patulin; fullerenol quantum dots; aptasensor; fluorescence

期刊名称：TOXINS （ 影响因子：5.075； 五年影响因子：5.305 ）

ISSN：

年卷期： 2022 年 14 卷 4 期

页码：

收录情况： SCI

摘要： A highly selective and sensitive aptasensor for detecting patulin (PAT) was constructed based on the fluorescence quenching of fullerenol quantum dots (FOQDs) towards carboxytetramethylrhodamine (TAMRA) through PET mechanism. The pi-pi stacking interaction between PAT aptamer and FOQDs closed the distance between TAMRA and FOQDs and the fluorescence of TAMRA was quenched with maximum quenching efficiency reaching 85%. There was no non-specific fluorescence quenching caused by FOQDs. In the presence of PAT, the PAT aptamer was inclined to bind with PAT and its conformation was changed. Resulting in the weak pi-pi stacking interaction between PAT aptamer and FOQDs. Therefore, the fluorescence of TAMRA recovered and was linearly correlated to the concentration of PAT in the range of 0.02-1 ng/mL with a detection limit of 0.01 ng/mL. This PAT aptasensor also performed well in apple juice with linear dynamic range from 0.05-1 ng/mL. The homogeneous fluorescence aptasensor shows broad application prospect in the detection of various food pollutants.

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