Mulberry leaf polysaccharides ameliorate lipid metabolic disorders in high-fat diet-fed Megalobrama amblycephala: changes in intestinal transcriptome, metabolome, and microbiota

文献类型： 外文期刊

第一作者： Jiang, Wenqiang

作者： Jiang, Wenqiang;Gu, Zhengyan;Lu, Siyue;Lin, Yan;Miao, Linghong;Ge, Xianping;Jiang, Wenqiang;Qian, Linjie;Mu, Qiaoqiao;Miao, Linghong;Ge, Xianping

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关键词： Mulberry leaf polysaccharides; High fat metabolism; Multi-omics combined analysis

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.5； 五年影响因子：8.7 ）

ISSN： 0141-8130

年卷期： 2025 年 320 卷

页码：

收录情况： SCI

摘要： This study explored the potential protective role of mulberry leaf polysaccharides (MLPs) in mitigating intestinal damage induced by a high-fat (HF) diet in Megalobrama amblycephala juveniles. This was achieved through comprehensive multi-omics analysis on transcriptome, metabolome, and intestinal microbiota. Results showed that significant intestinal lipid deposition and endoplasmic reticulum (ER) stress were observed in juveniles of the HF group (P < 0.05). A multi-omics further analysis between the HF group and HF-MLP group (supplementing 1.00 g MLPs/kg in HF diet) was conducted. Transcriptomic analysis identified significant differential expression genes (DEGs) enrichment in the ER function and lipid metabolism pathways (P < 0.05). MLPs supplementation significantly decreased expression levels of ER stress markers (bip, chop) induced by triglyceride accumulation in HF diet-fed juverniles, likely through the suppression of srebf1 (P < 0.05). It further reduced lipid peroxidation and improved intestinal antioxidant capacity (P < 0.05). MLPs supplementation restored intestinal barrier integrity and downregulated tlr4/nf-kappa b pathway-related genes expressions (P < 0.05). Metabolomic analysis revealed that MLPs restored ER membrane function and preserved cell membrane integrity by reducing phosphatidylcholine, acylcarnitine, and lysophosphatidylethanolamine levels (P < 0.05). Furthermore, MLPs counteracted HF diet-induced intestinal dysbiosis by increasing the abundances of Muribaculaceae and Bacteroidales. In conclusion, MLPs supplementation regulated intestinal microbiota composition, alleviated ER stress caused by lipid accumulation, and reduced lipid peroxidation, decreased intestinal inflammation in highfat diet- fed M. amblycephala juverniles.

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