LncRNA-TBP mediates TATA-binding protein recruitment to regulate myogenesis and induce slow-twitch

文献类型： 外文期刊

第一作者： Ma, Manting

作者： Ma, Manting;Cai, Bolin;Zhou, Zhen;Kong, Shaofen;Zhang, Jing;Xu, Haiping;Zhang, Xiquan;Nie, Qinghua;Ma, Manting;Cai, Bolin;Zhou, Zhen;Kong, Shaofen;Zhang, Jing;Xu, Haiping;Zhang, Xiquan;Nie, Qinghua;Ma, Manting;Cai, Bolin;Zhou, Zhen;Kong, Shaofen;Zhang, Jing;Xu, Haiping;Zhang, Xiquan;Nie, Qinghua;Ma, Manting;Cai, Bolin;Zhou, Zhen;Kong, Shaofen;Zhang, Jing;Xu, Haiping;Zhang, Xiquan;Nie, Qinghua

作者机构：

关键词： LncRNA-TBP; RNA binding proteins (RBPs); TBP; Myogenesis; Muscle phenotype transformation

期刊名称：CELL COMMUNICATION AND SIGNALING （ 影响因子：8.4； 五年影响因子：8.0 ）

ISSN：

年卷期： 2023 年 21 卷 1 期

页码：

收录情况： SCI

摘要： Background Skeletal muscle is comprised of heterogeneous myofibers that differ in their physiological and metabolic parameters. Of these, slow-twitch (type I; oxidative) myofibers have more myoglobin, more mitochondria, and higher activity of oxidative metabolic enzymes compared to fast-twitch (type II; glycolytic) myofibers.Methods In our previous study, we found a novel LncRNA-TBP (for "LncRNA directly binds TBP transcription factor ") is specifically enriched in the soleus (which has a higher proportion of slow myofibers). The primary myoblast cells and animal model were used to assess the biological function of the LncRNA-TBP in vitro or in vivo. Meanwhile, we performed a RNA immunoprecipitation (RIP) and pull-down analysis to validate this interaction between LncRNA-TBP and TBP.Results Functional studies demonstrated that LncRNA-TBP inhibits myoblast proliferation but promotes myogenic differentiation in vitro. In vivo, LncRNA-TBP reduces fat deposition, activating slow-twitch muscle phenotype and inducing muscle hypertrophy. Mechanistically, LncRNA-TBP acts as a regulatory RNA that directly interacts with TBP protein to regulate the transcriptional activity of TBP-target genes (such as KLF4, GPI, TNNI2, and CDKN1A).Conclusion Our findings present a novel model about the regulation of LncRNA-TBP, which can regulate the transcriptional activity of TBP-target genes by recruiting TBP protein, thus modulating myogenesis progression and inducing slow-twitch fibers.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序