A Novel Phytase Derived from an Acidic Peat-Soil Microbiome Showing High Stability under Acidic Plus Pepsin Conditions

文献类型： 外文期刊

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关键词： Phytase; Metagenomic; Acidobacteria; Gastric stable; Wide pH profile; Wide substrate profile

期刊名称：JOURNAL OF MOLECULAR MICROBIOLOGY AND BIOTECHNOLOGY （ 影响因子：0.803； 五年影响因子：1.609 ）

ISSN： 1464-1801

年卷期： 2016 年 26 卷 4 期

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收录情况： SCI

摘要： Four novel phytases of the histidine acid phosphatase family were identified in two publicly available metagenomic datasets of an acidic peat-soil microbiome in northeastern Bavaria, Germany. These enzymes have low similarity to all the reported phytases. They were overexpressed in Escherichia coli and purified. Catalytic efficacy in simulated gastric fluid was measured and compared among the four candidates. The phytase named rPhyPt4 was selected for its high activity. It is the first phytase identified from unculturable Acidobacteria. The phytase showed a longer half-life than all the gastric-stable phytases that have been reported to date, suggesting a strong resistance to low pH and pepsin. A wide pH profile was observed between pH 1.5 and 5.0. At the optimum pH (2.5) the activity was 2,790 mu mol/min/mg at the physiological temperature of 37 degrees C and 3,989 mu mol/min/mg at the optimum temperature of 60 degrees C. Due to the competent activity level as well as the high gastric stability, the phytase could be a potential candidate for practical use in livestock and poultry feeding (C) 2016 S. Karger AG, Basel

分类号： Q93`Q81

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