Characterization of an Escherichia coli Isolate Coharboring the Virulence Gene astA and Tigecycline Resistance Gene tet(X4) from a Dead Piglet

文献类型： 外文期刊

第一作者： Wang, Jianmei

作者： Wang, Jianmei;Huang, Yuting;Guan, Chunjiu;Yang, Hua;Ma, Jiangang;Tang, Biao;Wang, Jianmei;Huang, Yuting;Guan, Chunjiu;Yang, Hua;Ma, Jiangang;Tang, Biao;Huang, Yuting;Guan, Chunjiu;Liu, Canying;Li, Jie;Zhao, Guoping

作者机构：

关键词： Escherichia coli; tet(X4); astA; complete genome sequence

期刊名称：PATHOGENS （ 影响因子：3.7； 五年影响因子：3.7 ）

ISSN：

年卷期： 2023 年 12 卷 7 期

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收录情况： SCI

摘要： tet(X4) is the critical resistance gene for tigecycline degradation that has been continually reported in recent years. In particular, pathogenic bacteria carrying tet(X4) are a severe threat to human health. However, information describing Escherichia coli coharboring tet(X4) with virulence genes is limited. Here, we isolated an E. coli strain coharboring tet(X4) and the heat-stable toxin gene astA from a dead piglet. The strain named 812A1-131 belongs to ST10. The genome was sequenced using the Nanopore and Illumina platforms. The virulence genes astA and tet(X4) are located on the chromosome and in the IncHI1-type plasmid p812A1-tetX4-193K, respectively. The plasmid could be conjugatively transferred to recipient E. coli J53 with high frequency. In vivo experiments showed that strain 812A1-131 is pathogenic to Galleria mellonella and could colonize the intestines of mice. In summary, pathogenic E. coli could receive a plasmid harboring the tet(X4) gene, which can increase the difficulty of treatment. The prevalence and transmission mechanisms of pathogenic bacteria coharboring the tet(X4) gene need more attention.

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